Several lines of evidence indicate that antibody responses to T-dependent antigens require complement receptors expressed on either B lymphocytes or follicular dendritic cells. We have used RAG-2 deficient blastocyst complementation to create mice specifically lacking B cell complement receptors. Despite normal expression of complement receptor 1 (CR1[CD35]) and CR2 (CD21) on follicular dendritic cells, these mice have a profound defect in their capacity to mount a T-dependent antibody response. This is the first direct demonstration in vivo that B cell expression of complement receptors is required for a humoral immune response. This is the first direct demonstration in vivo that B cell expression of complement receptors is required for a humoral immune response. This suggests that CD21 and/or CD35 on B lymphocytes may be required for cellular activation, adsorptive endocytosis of antigen, recruitment to germinal centers, and/or protection from apoptosis during the humoral response to T-dependent antigens.
Article|
April 01 1996
Antibody response to a T-dependent antigen requires B cell expression of complement receptors.
D A Croix,
D A Croix
Graduate Program in Immunology, Johns Hopkins School of Medicine, Baltimore, Maryland 21205-2196, USA.
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J M Ahearn,
J M Ahearn
Graduate Program in Immunology, Johns Hopkins School of Medicine, Baltimore, Maryland 21205-2196, USA.
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A M Rosengard,
A M Rosengard
Graduate Program in Immunology, Johns Hopkins School of Medicine, Baltimore, Maryland 21205-2196, USA.
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S Han,
S Han
Graduate Program in Immunology, Johns Hopkins School of Medicine, Baltimore, Maryland 21205-2196, USA.
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G Kelsoe,
G Kelsoe
Graduate Program in Immunology, Johns Hopkins School of Medicine, Baltimore, Maryland 21205-2196, USA.
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M Ma,
M Ma
Graduate Program in Immunology, Johns Hopkins School of Medicine, Baltimore, Maryland 21205-2196, USA.
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M C Carroll
M C Carroll
Graduate Program in Immunology, Johns Hopkins School of Medicine, Baltimore, Maryland 21205-2196, USA.
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D A Croix
Graduate Program in Immunology, Johns Hopkins School of Medicine, Baltimore, Maryland 21205-2196, USA.
J M Ahearn
Graduate Program in Immunology, Johns Hopkins School of Medicine, Baltimore, Maryland 21205-2196, USA.
A M Rosengard
Graduate Program in Immunology, Johns Hopkins School of Medicine, Baltimore, Maryland 21205-2196, USA.
S Han
Graduate Program in Immunology, Johns Hopkins School of Medicine, Baltimore, Maryland 21205-2196, USA.
G Kelsoe
Graduate Program in Immunology, Johns Hopkins School of Medicine, Baltimore, Maryland 21205-2196, USA.
M Ma
Graduate Program in Immunology, Johns Hopkins School of Medicine, Baltimore, Maryland 21205-2196, USA.
M C Carroll
Graduate Program in Immunology, Johns Hopkins School of Medicine, Baltimore, Maryland 21205-2196, USA.
Online ISSN: 1540-9538
Print ISSN: 0022-1007
J Exp Med (1996) 183 (4): 1857–1864.
Citation
D A Croix, J M Ahearn, A M Rosengard, S Han, G Kelsoe, M Ma, M C Carroll; Antibody response to a T-dependent antigen requires B cell expression of complement receptors.. J Exp Med 1 April 1996; 183 (4): 1857–1864. doi: https://doi.org/10.1084/jem.183.4.1857
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