The production of class-switched antibodies, particularly immunoglobulin (Ig) G1 and IgE, occurs efficiently in T cell receptor (TCR) alpha-/- mice that are congenitally devoid of alpha/beta T cells. This finding runs counter to a wealth of data indicating that IgG1 and IgE synthesis are largely dependent on the collaboration between B and alpha/beta T cells. Furthermore, many of the antibodies synthesized in TCR alpha-/- mice are reactive to a similar spectrum of self-antigens as that targeted by autoantibodies characterizing human systemic lupus erythematosus (SLE). SLE, too, is most commonly regarded as an alpha/beta T cell-mediated condition. To distinguish whether the development of autoantibodies in TCR alpha-/- mice is due to an intrinsic de-regulation of B cells, or to a heretofore poorly characterized collaboration between B and "non-alpha/beta T" cells, the phenotype has been reconstituted by transfer of various populations of B and non-alpha/beta T cells including cloned gamma/delta T cells derived from TCR alpha-/- mice, to severe combined immunodeficient (SCID) mice. The results establish that the reproducible production of IgG1 (including autoantibodies) is a product of non-alpha/beta T cell help that can be provided by gamma/delta T cells. This type of B-T collaboration sustains the production of germinal centers, lymphoid follicles that ordinarily are anatomical signatures of alpha/beta T-B cell collaboration. Thus, non-alpha/beta T cell help may drive Ig synthesis and autoreactivity under various circumstances, especially in cases of alpha/beta T cell immunodeficiency.
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May 01 1996
Germinal center formation, immunoglobulin class switching, and autoantibody production driven by "non alpha/beta" T cells.
L Wen,
L Wen
Department of Biology, Yale University, New Haven, Connecticut 06520, USA.
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W Pao,
W Pao
Department of Biology, Yale University, New Haven, Connecticut 06520, USA.
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F S Wong,
F S Wong
Department of Biology, Yale University, New Haven, Connecticut 06520, USA.
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Q Peng,
Q Peng
Department of Biology, Yale University, New Haven, Connecticut 06520, USA.
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J Craft,
J Craft
Department of Biology, Yale University, New Haven, Connecticut 06520, USA.
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B Zheng,
B Zheng
Department of Biology, Yale University, New Haven, Connecticut 06520, USA.
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G Kelsoe,
G Kelsoe
Department of Biology, Yale University, New Haven, Connecticut 06520, USA.
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L Dianda,
L Dianda
Department of Biology, Yale University, New Haven, Connecticut 06520, USA.
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M J Owen,
M J Owen
Department of Biology, Yale University, New Haven, Connecticut 06520, USA.
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A C Hayday
A C Hayday
Department of Biology, Yale University, New Haven, Connecticut 06520, USA.
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L Wen
Department of Biology, Yale University, New Haven, Connecticut 06520, USA.
W Pao
Department of Biology, Yale University, New Haven, Connecticut 06520, USA.
F S Wong
Department of Biology, Yale University, New Haven, Connecticut 06520, USA.
Q Peng
Department of Biology, Yale University, New Haven, Connecticut 06520, USA.
J Craft
Department of Biology, Yale University, New Haven, Connecticut 06520, USA.
B Zheng
Department of Biology, Yale University, New Haven, Connecticut 06520, USA.
G Kelsoe
Department of Biology, Yale University, New Haven, Connecticut 06520, USA.
L Dianda
Department of Biology, Yale University, New Haven, Connecticut 06520, USA.
M J Owen
Department of Biology, Yale University, New Haven, Connecticut 06520, USA.
A C Hayday
Department of Biology, Yale University, New Haven, Connecticut 06520, USA.
Online ISSN: 1540-9538
Print ISSN: 0022-1007
J Exp Med (1996) 183 (5): 2271–2282.
Citation
L Wen, W Pao, F S Wong, Q Peng, J Craft, B Zheng, G Kelsoe, L Dianda, M J Owen, A C Hayday; Germinal center formation, immunoglobulin class switching, and autoantibody production driven by "non alpha/beta" T cells.. J Exp Med 1 May 1996; 183 (5): 2271–2282. doi: https://doi.org/10.1084/jem.183.5.2271
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