To investigate the role of TNF alpha in the development of in vivo immune response we have generated TNF alpha-deficient mice by gene targeting. Homozygous mutant mice are viable and fertile, develop lymph nodes and Peyer's patches and show no apparent phenotypic abnormalities, indicating that TNF alpha is not required for normal mouse development. In the absence of TNF alpha mice readily succumb to L. monocytogenes infections and show reduced contact hypersensitivity responses. Furthermore, TNF alpha knockout mice are resistant to the systemic toxicity of LPS upon D-galactosamine sensitization, yet they remain sensitive to high doses of LPS alone. Most interestingly, TNF alpha knockout mice completely lack splenic primary B cell follicles and cannot form organized follicular dendritic cell (FDC) networks and germinal centers. However, despite the absence of B cell follicles, Ig class-switching can still occur, yet deregulated humoral immune responses against either thymus-dependent (TD) or thymus-independent (TI) antigens are observed. Complementation of TNF alpha functioning by the expression of either human or murine TNF alpha transgenes is sufficient to reconstitute these defects, establishing a physiological role for TNF alpha in regulating the development and organization of splenic follicular architecture and in the maturation of the humoral immune response.
Article|
October 01 1996
Immune and inflammatory responses in TNF alpha-deficient mice: a critical requirement for TNF alpha in the formation of primary B cell follicles, follicular dendritic cell networks and germinal centers, and in the maturation of the humoral immune response.
M Pasparakis,
M Pasparakis
Department of Molecular Genetics, Hellenic Pasteur Institute, Athens, Greece.
Search for other works by this author on:
L Alexopoulou,
L Alexopoulou
Department of Molecular Genetics, Hellenic Pasteur Institute, Athens, Greece.
Search for other works by this author on:
V Episkopou,
V Episkopou
Department of Molecular Genetics, Hellenic Pasteur Institute, Athens, Greece.
Search for other works by this author on:
G Kollias
G Kollias
Department of Molecular Genetics, Hellenic Pasteur Institute, Athens, Greece.
Search for other works by this author on:
M Pasparakis
Department of Molecular Genetics, Hellenic Pasteur Institute, Athens, Greece.
L Alexopoulou
Department of Molecular Genetics, Hellenic Pasteur Institute, Athens, Greece.
V Episkopou
Department of Molecular Genetics, Hellenic Pasteur Institute, Athens, Greece.
G Kollias
Department of Molecular Genetics, Hellenic Pasteur Institute, Athens, Greece.
Online ISSN: 1540-9538
Print ISSN: 0022-1007
J Exp Med (1996) 184 (4): 1397–1411.
Citation
M Pasparakis, L Alexopoulou, V Episkopou, G Kollias; Immune and inflammatory responses in TNF alpha-deficient mice: a critical requirement for TNF alpha in the formation of primary B cell follicles, follicular dendritic cell networks and germinal centers, and in the maturation of the humoral immune response.. J Exp Med 1 October 1996; 184 (4): 1397–1411. doi: https://doi.org/10.1084/jem.184.4.1397
Download citation file: