Thymic selection of natural killer-1+ natural T cells that express alpha beta T cell receptors requires a conserved beta 2-microglobulin-associated molecule, presumably CD1d, displayed by CD4+8+ thymocytes. Here we demonstrate that positive selection of natural T cells occurs independent of transporters associated with antigen presentation-1 (TAP-1) function. Moreover, natural T cells in TAP-1o/o mice are numerically expanded. Several H-2 class Ib molecules function in a TAP-independent manner, suggesting that if expressed in TAP-1o/o thymocytes, they could play a role in natural T cell development. Of these class Ib molecules, H-2TL is expressed by TAP-1o/o thymocytes. Moreover, we find that thymi of TL+ mice congenic or transgenic for H-2T18 also have a numerically expanded natural T cell repertoire compared with TL- mice. This expansion, as in TAP-1o/o thymi, is evident in each of the limited T cell receptor V beta chains expressed by natural T cells, suggesting that TL and CD1d impact similar repertoires. Thus TL, in addition to CD1d, plays a role in natural T cell development.
Article|
October 01 1996
Expansion of natural (NK1+) T cells that express alpha beta T cell receptors in transporters associated with antigen presentation-1 null and thymus leukemia antigen positive mice.
S Joyce,
S Joyce
Department of Microbiology & Immunology, Pennsylvania State University College of Medicine, Milton S. Hershey Medical Center, Hershey 17033, USA.
Search for other works by this author on:
I Negishi,
I Negishi
Department of Microbiology & Immunology, Pennsylvania State University College of Medicine, Milton S. Hershey Medical Center, Hershey 17033, USA.
Search for other works by this author on:
A Boesteanu,
A Boesteanu
Department of Microbiology & Immunology, Pennsylvania State University College of Medicine, Milton S. Hershey Medical Center, Hershey 17033, USA.
Search for other works by this author on:
A D DeSilva,
A D DeSilva
Department of Microbiology & Immunology, Pennsylvania State University College of Medicine, Milton S. Hershey Medical Center, Hershey 17033, USA.
Search for other works by this author on:
P Sharma,
P Sharma
Department of Microbiology & Immunology, Pennsylvania State University College of Medicine, Milton S. Hershey Medical Center, Hershey 17033, USA.
Search for other works by this author on:
M J Chorney,
M J Chorney
Department of Microbiology & Immunology, Pennsylvania State University College of Medicine, Milton S. Hershey Medical Center, Hershey 17033, USA.
Search for other works by this author on:
D Y Loh,
D Y Loh
Department of Microbiology & Immunology, Pennsylvania State University College of Medicine, Milton S. Hershey Medical Center, Hershey 17033, USA.
Search for other works by this author on:
L Van Kaer
L Van Kaer
Department of Microbiology & Immunology, Pennsylvania State University College of Medicine, Milton S. Hershey Medical Center, Hershey 17033, USA.
Search for other works by this author on:
S Joyce
Department of Microbiology & Immunology, Pennsylvania State University College of Medicine, Milton S. Hershey Medical Center, Hershey 17033, USA.
I Negishi
Department of Microbiology & Immunology, Pennsylvania State University College of Medicine, Milton S. Hershey Medical Center, Hershey 17033, USA.
A Boesteanu
Department of Microbiology & Immunology, Pennsylvania State University College of Medicine, Milton S. Hershey Medical Center, Hershey 17033, USA.
A D DeSilva
Department of Microbiology & Immunology, Pennsylvania State University College of Medicine, Milton S. Hershey Medical Center, Hershey 17033, USA.
P Sharma
Department of Microbiology & Immunology, Pennsylvania State University College of Medicine, Milton S. Hershey Medical Center, Hershey 17033, USA.
M J Chorney
Department of Microbiology & Immunology, Pennsylvania State University College of Medicine, Milton S. Hershey Medical Center, Hershey 17033, USA.
D Y Loh
Department of Microbiology & Immunology, Pennsylvania State University College of Medicine, Milton S. Hershey Medical Center, Hershey 17033, USA.
L Van Kaer
Department of Microbiology & Immunology, Pennsylvania State University College of Medicine, Milton S. Hershey Medical Center, Hershey 17033, USA.
Online ISSN: 1540-9538
Print ISSN: 0022-1007
J Exp Med (1996) 184 (4): 1579–1584.
Citation
S Joyce, I Negishi, A Boesteanu, A D DeSilva, P Sharma, M J Chorney, D Y Loh, L Van Kaer; Expansion of natural (NK1+) T cells that express alpha beta T cell receptors in transporters associated with antigen presentation-1 null and thymus leukemia antigen positive mice.. J Exp Med 1 October 1996; 184 (4): 1579–1584. doi: https://doi.org/10.1084/jem.184.4.1579
Download citation file: