Binding of the T cell receptor (TCR) to a bacterial superantigen (SAG) results in stimulation of a large population of T cells and subsequent inflammatory reactions. To define the functional contribution of TCR residues to SAG recognition, binding by 24 single-site alanine substitutions in the TCR Vβ domain to Staphylococcus aureus enterotoxin (SE) C3 was measured, producing an energy map of the TCR–SAG interaction. The results showed that complementarity determining region 2 (CDR2) of the Vβ contributed the majority of binding energy, whereas hypervariable region 4 (HV4) and framework region 3 (FR3) contributed a minimal amount of energy. The crystal structure of the Vβ8.2–SEC3 complex suggests that the CDR2 mutations act by disrupting Vβ main chain interactions with SEC3, perhaps by affecting the conformation of CDR2. The finding that single Vβ side chain substitutions had significant effects on binding and that other SEC3-reactive Vβ are diverse at these same positions indicates that SEC3 binds to other TCRs through compensatory mechanisms. Thus, there appears to be strong selective pressure on SAGs to maintain binding to diverse T cells.
A T cell recognizes ligand through the interaction of its α/β TCR and an antigenic peptide expressed in the context of an MHC (pMHC). This process of MHC restriction provides for the specificity of T cell responses to pathogens. Superantigens (SAGs) are proteins of viral or bacterial origin that stimulate T cell populations by binding and cross-linking the TCR with a class II MHC product, thus circumventing the normal recognition of intracellularly processed pMHC 1. Because SAGs can bind to Vβ regions directly, SAG engagement of T cells leads to polyclonal expansion of up to 20% of all T cells. In contrast, normal pMHC antigen recognition might involve the stimulation of as few as only 1 in 105 or 106 T cells 2. SAG-mediated recognition can lead to hyperactive responses associated with the release of T cell cytokines or to T cell deletion and anergy 3,4. It has also been suggested that SAG-mediated effects may contribute to particular autoimmune and inflammatory disorders 3.
Two classes of SAGs have been identified: endogenous retroviral-encoded cellular-bound proteins, such as mouse mammary tumor virus (MMTV)-encoded Mls antigen, and exogenous soluble proteins secreted from bacteria 4,5,6. The latter SAGs include Mycoplasma arthritidis mitogen 7,8, streptococcal pyrogenic exotoxins 9,10,11, staphylococcal toxic shock syndrome toxin 1, enterotoxins, and exfoliative toxins 1,9. Most structural and biological activity studies have focused on the SAGs of Staphylococcus aureus, which have been associated with food poisoning and toxic shock syndrome 3.
The S. aureus enterotoxins (SEs) exist in isoforms A–E and G–I 12, which bind to numerous Vβ families of murine and human origin 1. The structures of SEs to date reveal a conserved architecture, consisting of a small NH2-terminal β-barrel domain and a large COOH-terminal domain that contains a β-grasp motif, connected by a long solvent-accessible α helix 2. Early studies suggested that the SE-binding site of the TCR was positioned away from the conventional pMHC combining site 13. The Vβ cross-reactivity possessed by SEs suggested that the proteins bound to conserved structural elements of the TCR shared among different Vβ regions 1. Mutagenesis studies indicated that CDR2, and perhaps CDR1 to a lesser degree, were involved in the reactivity with SEB and SEA 14.
The recent crystallographic structures of SEC3 and SEB in complex with the Vβ8.2 region of the mouse TCR 14.3.d 15,16 revealed that CDR2, hypervariable region 4 (HV4), and framework region 3 (FR3) contact these SAGs. In both complexes, CDR2 occupied the cleft between the large and small domains of SEC3 and shared multiple contacts and hydrogen bonds with each domain 17. CDR2 contained the majority of surface contacts for SEC3 (63%) and for SEB (50%), and these contacts involved exclusively Vβ main chain atoms. The FR3 region of the Vβ domain contributed 32 and 34% of the contacts with SEC3 and SEB, whereas HV4 provided 7 and 9% of the contacts with SEC3 and SEB 16. Direct contacts with CDR1 and CDR3 were not observed in either complex. Therefore, SE binding to the TCR incorporates regions of the TCR that are essential in pMHC binding (CDR2), while also involving regions that are not important in pMHC recognition (FR3 and HV4).
SAGs stimulate T cells not just by binding to the TCR, but by binding a class II MHC product through a different face of the SAG molecule. This TCR–SAG–pMHC ternary complex brings a T cell together with a class II+ cell and it presumably leads to TCR clustering at the T cell surface. The stoichiometry of these interactions has yet to be fully determined and may vary among the different SAGs 12. Nevertheless, recent evidence showed that the Vα of the TCR appears to be involved in binding to the class II MHC product and thereby increasing the stability of the complexes 18. This finding is consistent with the initial work of Blackman and colleagues and more recently from other groups that suggested that SAG-mediated effects may involve CDR3 of the β chain and the Vα region 8,19,20,21,22,23,24,25. The latter conclusions were based on evidence of restrictions in either CDR3 residues or Vα regions that were expressed by a specific Vβ+ population that reacted with an SAG.
Although the structure of individual components and the complexes have provided a view of the contact residues, mutagenesis studies can provide a quantitative view of the residues that are of importance from the energetic perspective. These interactions usually represent a subset of the interface residues identified as contacts in the crystal structure 26. However, even the same protein surface can involve either only a few very important residues in ligand binding (e.g., the antibody D1.3 in its interaction with hen egg lysozyme) or a larger number of moderately important residues (e.g., the D1.3 interaction with its antiidiotype antibody E5.2) 27. This possibility is important with regard to Vβ–SEC3 interactions, as it is possible that the majority of the energy could be derived from only a few contacts and these contacts might be conserved among the different SEC3-reactive Vβ regions.
To define the functional contribution of individual TCR residues toward SEC3 recognition, we have used alanine scanning mutagenesis of a Vβ8.2 TCR from the T cell clone 2C. Due to the relatively low affinity of the SEC3–Vβ8.2 TCR interaction (equilibrium binding constant [KD] > 10 μM), an affinity-matured SEC3 variant (KD ∼ 180 nM) was used to facilitate the mutagenic binding studies. Residues in CDR1 and CDR2, and to a lesser degree HV4 and FR3, served as energetic hotspots for binding to the SEC3 variant. Based on the structure of the Vβ8–SEC3 complex 16, most of the CDR1 energy was predicted to be involved in binding the region of the SEC3 variant that had been mutated to yield higher affinity. Thus, CDR2 contributed the majority of the energy to the interaction of Vβ8.2 and the wild-type (wt) SEC3. HV4 and FR3 contributed less energy than would be predicted from their contribution to the total contact area of the complex. The observation that CDR2 mutations affected binding to SEC3 is somewhat surprising in the sense that all of the contacts found at the CDR2–SEC3 interface involved Vβ main chain interactions 16. Side chain substitutions could be affecting these main chain interactions through conformational changes in the Vβ8 backbone.
As other Vβ regions that interact with SEC3 exhibit even more amino acid variability at the energetically important positions (i.e., than a single alanine mutation), there appears to be extensive diversity at the molecular level in the binding of SEC3 to different Vβ regions. Thus, from a consideration of binding energies the results provide evidence that the same SAG may require compensatory interactions among different Vβ regions. These interactions could involve other residues within the Vβ (i.e., residues different from those important in the Vβ8.2–SEC3 interaction) or residues within CDR3 loops or Vα regions that act by contacting and stabilizing the interaction with class II MHC. In light of the minimal binding energy differences that are known to elicit agonist versus antagonist responses 28,29, the results also suggest how the same SAG could yield widely disparate biological affects, depending on the Vβ region involved.
Materials And Methods
Alanine-substituted single-chain (sc)TCRs were constructed as thioredoxin fusion proteins with a His6 tag using two mutagenesis methods. First, a PCR-based technique 30 used a short mutagenic primer and a Vβ-specific primer to generate a “megaprimer,” which was isolated and used in a second PCR with the opposing Vα-specific primer. All PCRs were carried out using cloned Pfu DNA polymerase (Stratagene). The KpnI/BamHI-digested PCR product was ligated into a pUC-19M vector, transformed into Escherichia coli strain DH5-α for sequencing. The scTCR inserts were then subcloned into the pTRXFus vector (Invitrogen) and transformed into E. coli strain GI698 for expression. Alternatively, “QuikChange” site-directed mutagenesis (Stratagene) was used to mutagenize the wt-scTCR-pTRXFus. The DpnI-digested product was transformed into E. coli strain GI698 for sequencing and expression.
TCR Expression and Purification.
Proteins were expressed in E. coli strain GI698 as described previously 31 and purified from inclusion bodies by denaturing affinity chromatography using His-Bind resin (Novagen). After renaturation by dialysis 31, the monomeric proteins were purified by Superdex 200 gel filtration (Amersham Pharmacia Biotech) using HPLC.
KJ16 32 is a rat IgG mAb specific for mouse Vβ8.1 and Vβ8.2 regions of the TCR. F23.1 33 is a mouse IgG2a mAb specific for mouse Vβ8.1, Vβ8.2, and Vβ8.3 regions. F23.2 33 is a mouse IgG1 mAb specific for the mouse Vβ8.2 region. Antibodies were purified from either culture supernatants or ascites, followed by protein G– and protein A–agarose (GIBCO BRL) column chromatography for KJ16 and F23.1/F23.2, respectively.
SEC3 variant 1A4 (SEC3-1A4) was isolated from a phage display library (Anderson, P.S., and R.A. Mariuzza, manuscript in preparation). Soluble protein was prepared as described previously 18. In brief, bacterial expression was induced at mid-log density by the addition of isopropyl-β-d-thiogalactopyranoside (IPTG) and continued for 4 h. The protein was purified from the periplasmic fraction using a RedA dye–agarose matrix (Amicon), followed by chromatography using a monoQ column (Amersham Pharmacia Biotech).
Vβ8-specific competition ELISAs were performed by adsorption of KJ16, F23.1, or F23.2 to the wells of Immulon 2HB analytical plates (Dynatech Labs). The wells were blocked with PBS that contained 0.25% BSA and 0.05% Tween 20. After washing, 100 μl of mutant or wt-scTCR and 50 μl of biotinylated wt-scTCR were added to the wells for 1 h The wells were then washed, and binding was detected using horseradish peroxidase (HRP)-streptavidin followed by tetramethylbenzidine (TMB) peroxidase substrate (Kirkegaard & Perry). The relative half-maximal inhibitory concentration (IC50) for each mutant relative to wt was calculated using linear regression analysis.
SEC3-1A4 competition ELISAs were performed by adsorption of wt-scTCR to the wells of the analytical plates. The wells were blocked and after washing, 50 μl of mutant or wt-scTCR and 50 μl of biotinylated SEC3-1A4 were added to the wells. After 1 h, the wells were washed and binding was detected using HRP-streptavidin followed by TMB substrate. The relative IC50 for each mutant relative to wt was calculated using linear regression analysis. SEC3-1A4 reactivity was normalized for Vβ8 reactivity to account for variations in the percentages of properly folded scTCRs.
Surface plasmon resonance (SPR) studies were performed using a BIAcore 1000® instrument. Binding experiments were as described previously 17,18. SEC3-1A4 was immobilized on the sensor chip, and various concentrations of scTCR were allowed to bind until equilibrium was reached (1–5 min). The response, corrected for nonspecific binding and aggregation 34, was used to determine the equilibrium binding constant (KD) by Scatchard analysis.
Binding of 2C scTCR to SEC3 Wt and SEC3 Variant 1A4.
The TCR used here is derived from the murine CTL clone 2C 36, which recognizes the allogeneic MHC Ld 37 and the syngeneic MHC Kb 38. The 2C TCR contains the Vα3.1 region and the Vβ8.2 region. With the exception of CDR3β, this is the same Vβ expressed by the TCR 14.3.d that was crystallized as a complex with SEC3 15. The 2C TCR was produced as a single chain containing an NH2-terminal thioredoxin domain (for increased solubility), the Vβ region, a 24-residue linker, and the Vα region 31. The reactivity of the scTCR construct is similar to the native receptor for the allogeneic MHC ligand Ld 39,40 and its syngeneic MHC ligand Kb (Lee, P.U.Y., H.R.O. Churchill, and D.M. Krantz, unpublished results).
The mAbs KJ16, F23.1, and F23.2 32,33 recognize conformational epitopes on the Vβ8 region 41. KJ16 and F23.1 react with framework regions (FRs) of the Vβ8 domain while F23.2 reacts with CDR1 and CDR2 residues 42. To develop a sensitive, quantitative binding assay for TCR mutants, we made use of an SEC3 variant, 1A4, that has a higher affinity for the TCR. This SEC3 variant was isolated by phage display of a library of SEC3 mutants, followed by selection on immobilized 14.3.d β chain 18. The SEC3 library contained random variants at five residues (102Gly, 103Lys, 104Val, 105Thr, and 106Gly) of the disulfide loop within the small domain of SEC3. Variant 1A4 and wt SEC3 were examined for binding to the 2C scTCR in a competition assay and in BIAcore analyses. The competition assay involved immobilization of F23.2 or KJ16 on wells, followed by addition of biotinylated scTCR and streptavidin-HRP. SEC3 wt and 1A4 were titrated as unlabeled inhibitors of this reaction (Fig. 1), showing that 1A4 was an ∼290-fold better inhibitor. Results were similar with either F23.2 or KJ16 as the immobilized antibody (data not shown).
SPR (BIAcore) analyses were performed using titrations of the 2C scTCR with either wt SEC3 or 1A4 immobilized on the surface of a chip (see below and Fig. 6). Based on equilibrium binding titrations, the wt 2C scTCR displayed a KD of 180 ± 55 nM for the SEC3-1A4 variant, but the affinity for the wt SEC3 was not measurable (KD > 5 μM) at the concentrations of scTCR that could be tested (i.e., the solubility limit of the 2C scTCR). Thus, the affinity of SEC3-1A4 was at least 30-fold higher based on this assay method. Similar results were obtained for binding of wt SEC3 and SEC3-1A4 variant to immobilized 14.3.d TCR-α/β yielding KD values of 22 μM and 150 nM, respectively (data not shown). Together, these results showed that the SEC3-1A4 variant had sufficient affinity to perform a mutagenic analysis.
Characterization of 2C TCR Mutants.
To analyze the interface between the TCR and SEC3-1A4, 29 single-site alanine mutants of the 2C scTCR (Fig. 2) were expressed in E. coli and purified by Ni column chromatography and gel filtration. As Vβ residue 52 is alanine, this position was mutated to tryptophan. The reactivities of the KJ16 and F23.1 antibodies with the mutant scTCR provided a measure of the concentration of folded protein in the preparations of refolded scTCR. Monomeric scTCR mutants were evaluated for Vβ-specific mAb reactivity in a competition ELISA using biotinylated wt-scTCR. The degree of properly folded scTCRs was determined from inhibition curves, as the IC50 relative to wt-scTCR (Fig. 3). The average reactivity of KJ16 and F23.1 with the scTCR was used as a measure of the concentration of properly folded protein.
23 of the single-site mutants showed reactivity with the anti-Vβ antibodies, KJ16 and F23.1, indicating that they were properly folded. Seven mutants (Vβ residues 25βGln, 29βHis, 32βMet, 49βSer, 54βSer, 69βArg, and 75βPhe) had minimal or no mAb reactivity. Most of these are inaccessible residues in which the side chains are likely to be involved in the stability of the CDR or HV4 loops 35. Mutations in the three CDRs and HV4 could be evaluated using KJ16 and F23.1, as these loops are not part of the KJ16 or F23.1 epitopes. Because mutations in the FRs 57βLys and 66βLys might affect binding to KJ16 and/or F23.1, mAb F23.2 was also used as verification of proper refolding of these mutants. The Lys57βAla and Lys66βAla mutants bound to F23.2, KJ16, and F23.1 with reactivities similar to the wt-scTCR, indicating that these residues do not contribute energy to the epitopes of any of these Vβ8-specific mAbs and that the mutants were properly refolded.
Binding of Single-Site TCR Mutants to SEC3-1A4.
Binding affinities of the TCR mutants were also examined in two different assays. In one assay, an ELISA was performed with immobilized wt-scTCR followed by the addition of biotinylated SEC3-1A4 and streptavidin-HRP. The measurement of mutant scTCR affinities was evaluated by titrations with unlabeled scTCR to compete for SEC3 binding to the immobilized wt-scTCR. From the generation of binding inhibition curves (Fig. 4), the SEC3-1A4 reactivity was determined as the IC50 ratio of mutant to wt-scTCR. The average value of three or more independent assays was used to determine binding affinities, relative to wt-scTCR (Fig. 5). In the other assay, BIAcore analyses were performed with immobilized SEC3-1A4. Various concentrations of mutant scTCRs were allowed to reach binding equilibrium, and the KD of each mutant relative to wt-scTCR was determined by Scatchard analysis (43; Fig. 6). The values determined by the competition ELISA and SPR were completely consistent (Table). Only the reactivity values determined from the competition assay are summarized as the bar graph in Fig. 5.
Many alanine scanning studies have shown that only a few mutations at a subset of the contact residues result in large (>10-fold) reductions in binding. In the TCR scan, only 6 of the 25 TCR residues tested affected the binding of SEC3-1A4 by >10-fold (Fig. 5). These six residues were distributed among CDR1 (28βAsn, 30βAsn), CDR2 (51βGly, 52βAla, 52βGly), and HV4 (72βGln). Residues with significant yet more limited effects were also present in each of these loops and in the FR3 region (57βLys). Consistent with previous predictions that SAGs are largely Vβ specific, neither the βCDR3 loop nor the most prominent residue (100αPhe) at the surface of the pMHC binding site contributed binding energy to the SEC3–TCR interaction.
Although the most important residues shown in Fig. 5 are distributed throughout the linear sequence, the energy map of these residues in the three dimensional structure reveals an energetic hotspot centered around CDR2 residues 51–53 (Fig. 7). This hotspot is surrounded by residues in CDR1, HV4, and FR3 that contribute to the binding interaction. With the exception of CDR1, each of these regions has residues that are in contact with wt SEC3 in the 14.3.d Vβ–SEC3 complex. Analysis of the interface of the Vβ–SEC3 complex (Fig. 8) suggests that the important residues in CDR1 (28βAsn, 30βAsn) and HV4 residue 72βGln exert their effects by interacting with the region of SEC3 that has been altered in the 1A4 variant (shown in red on the small domain of SEC3). Accordingly, the binding energies measured for CDR1 residues 28βAsn and 30βAsn are likely contributing to the higher affinity associated with the Vβ8–SEC3-1A4 interaction. The energy associated with these three residues (ΔΔG ∼ 4.8 Kcal/mol) in the alanine scan could account for the 200–300-fold increase in affinity of the SEC3-1A4 variant. We expect that a structure of the mutant SEC3–TCR complex could help substantiate this prediction, but for the purpose of examining the wt SEC3–Vβ interaction, CDR1 residues 28βAsn and 30βAsn and HV4 residue 72βGln will not be considered further.
The CDR2 region of the Vβ domain of the TCR has the majority of structural contacts in the complex with SEC3 15,16. Residues 52βAla and 53βGly contact SEC3 residues 90Tyr and 210Gln, respectively, two residues that were previously identified as TCR-binding “hotspots” within SEC3 (Fig. 8; reference 17). The most significant reduction in binding was observed for residue 51βGly, which also contacts SEC3 but does not contact SEB 16. Accordingly, this position may in part account for the higher affinity of Vβ8.2 for SEC3 compared with SEB. Although the two adjacent CDR2 residues 50βTyr and 56βGlu contact SEC3 residues 91Val and 20Thr, respectively, mutation of these residues did not show significant effects on binding. Thus, like other protein–protein interactions, the energetic hotspot of the SEC3–Vβ8 interaction does not extend across the entire interface. Vβ residue 48Tyr, which is distal to the Vβ–SEC3 interface (Fig. 8), results in a moderate loss of binding when changed to alanine, probably because of effects this mutation has on the local conformation of the CDR2 loop and possibly on FR3 residue 57βLys. Unfortunately, the contribution of 54βSer, which contacts SEC3 residues 23Asn and 91Val, could not be determined since this alanine mutant failed to refold correctly.
In comparison to CDR2, the more limited influence of HV4 residues on the SEC3 binding energy was consistent with the observation that the HV4 loop possesses fewer contacts in the 14.3.d β chain–SEC3 complex than the CDR2 loop 16. Vβ residues 70βPro and 71βSer, which are on the proximal side of the HV4 loop and in contact with SEC3 residues 58Leu and 60Ser, contributed a moderate amount of binding energy. Mutation of residues on the distal side of the loop (73βGlu, 74βAsn, and 76βSer) did not reduce SEC3 binding and in fact alanine mutants of residues 73βGlu and 76βSer showed moderate increases in binding (see below). Residue 74βAsn is a possible glycosylation site in the native TCR. The observation that mutation of this position had no effect on SEC3 binding is consistent with previous findings that glycosylation of the TCR does not appear to affect SEC3 binding 43.
The two most accessible residues in Vβ FR3, 57βLys and 66βLys, are both in contact with SEC3 in the crystal structure. Mutation of 57βLys led to a moderate reduction in binding, probably due to the loss of multiple Van der Waals (vdw) interactions with SEC3 residues 19Gly and 20Thr. In contrast, alanine substitution of 66βLys led to a significant enhancement of binding (see below).
There were no direct contacts between CDR3 of 14.3.d TCR β chain and SEC3 observed in the crystal structure of the complex 15. Consistent with this finding, mutations at residues 97βGly, 105βThr, 106βLeu, and 107βTyr showed minimal effects on SEC3 binding. A small but consistent reduction in binding of the Gly97Ala mutation was observed in both the ELISA format (decrease of 1.4-fold) and by SPR (KD = 400 nM). This result may suggest that the CDR3 loop is in a position to effect in a moderate way the binding of this SAG. As indicated above, Vα residue 100αPhe is the most prominent residue on the pMHC-binding surface 35, yet it had no effect on binding SEC3. A recent study has shown that Vα CDR2 residues can affect binding to the SEC3–MHC complex but not to SEC3 alone 18.
Enhanced Binding of TCR Mutants to SEC3.
Most alanine scanning studies reveal a fraction of mutations that lead to slight increases in the binding of ligand 44. In the case of the TCR–SEC3 interaction, mutations at three TCR residues (66βLys, 73βGlu, and 76βSer) showed minor but significant increases in binding (Fig. 5). To determine if the combination of these mutations would yield further increases in affinity, all three residues were examined as double and triple mutants (Fig. 9). Each of the three double mutants exhibited affinities for the SEC3 that were above the single-site mutants. The triple mutant yielded the highest affinity, approximately fivefold greater than the wt-scTCR. The mechanism by which these modified side chains act to increase the affinity is unknown, but presumably the alanine mutations could: (a) eliminate steric interferences of the wt side chain, (b) stabilize the TCR in a conformation that recognizes the ligand, or (c) allow increased mobility of TCR regions that require such flexibility in the recognition of ligands. Our recent analyses of several TCR mutants that bind pMHC with higher affinity suggest that each of these possibilities might operate, depending on the residue, its location in the structure, and the ligand itself (45; and Lee, P.U.Y., H.R.O. Churchill, and D.M. Krantz, unpublished findings).
Alanine scanning mutagenesis has been used to characterize the functional contribution of individual residues in several protein–protein interactions, including antigen–antibody interactions 27,46 and TCR–SEC3 17. Since alanine substitution minimizes steric and/or electrostatic constraints on the tertiary structure of a protein, this approach provides a systematic analysis of protein binding thermodynamics 26,44. In this report, the relative contributions of Vβ residues in binding SEC3 were examined by alanine scanning mutagenesis. From these results, the energy of the TCR interface with SEC3 can be mapped as the change in binding free energy (ΔΔG) for each mutation (Table, and Fig. 5). The CDR2, CDR1, HV4, FR3, and CDR3 regions accounted for 59, 25, 11, 2, and 2%, respectively, of the 2C TCR binding energy toward the SEC3 variant 1A4. The mutated region of SEC3 in 1A4 is within the disulfide loop. This loop is believed to be highly flexible based on its poor resolution in crystal structures, even at 1.5 Å resolution 47. Least square superpositioning of structures of SEC3 and SEC3 in complex with the 14.3.d TCR β chain suggested that the loop could be very close to residues within the Vβ8 CDR1 and to HV4 residue 72βGln. Thus, we suspect that these residues affected binding to 1A4 because they are involved in contacts with the mutated SEC3 region.
With this in mind, the thermodynamic results shown here point to the significant energetic contribution of the CDR2 loop in SEC3 recognition by Vβ8.2 and they define this region as the hotspot of the TCR–SEC3 interaction. This hotspot is located at the tip of CDR2, and it involves predominantly residues 51βGly, 52βAla, and 53βGly. These are surrounded by a second shell of residues that appear to contribute a moderate amount of energy to the interaction: 55βThr, 57βLys, 70βPro, and 71βSer. Residue 54βSer is located within the same region but it was unable to be tested because the alanine mutant did not fold properly. The results are also consistent with the alanine scan that was performed from the SEC3 side of the interaction, in that most of the key residues in SEC3 are located within proximity to the key residues in the Vβ domain (Fig. 8; reference 17). It is important to point out that the exact energetic contributions of side chains within the CDR2 loop cannot be determined from this analysis. This is because analysis of the wt residues 51βGly, 52βAla, and 53βGly necessitated mutation to larger groups at the interface (51βAla, 52βTrp, and 53βAla, respectively), and these modifications may act by interfering with main chain interactions. Nevertheless, even more extensive changes are seen among other Vβ regions that are SEC3 reactive (Table; reference 3), suggesting that there are significant compensatory interactions that occur at TCR–SEC3–MHC interfaces (see below).
Direct comparison of the structures of the Vβ8.2 domain of the 14.3.d TCR and the corresponding domains of several other TCRs suggested a possible explanation for the Vβ-related specificity of SEC3 and SEB 12,16. The 14.3.d Vβ8.2 region and the SEC3-reactive human Vβ12.3 from the A6 TCR 48 showed an root mean squared (rms) difference of 0.9 Å when the 14 α-carbon atoms in the SEC3/SEB-binding site were superimposed. In contrast, the 14.3.d Vβ8.2 region and the SEC3-nonreactive mouse Vβ2.3 from the KB5-C20 TCR 49 showed an r.m.s difference of 3.0 Å for the same 14 α-carbon atoms in the SEC3/SEB-binding site. This significant difference in alignment is due primarily to the c′′ β strand hydrogen bonding to the outer β sheet d strand in Vβ2.3, instead of maintaining association with the c′ strand of its inner β sheet, as is the case with Vβ8.2 and Vβ12.3 16,49. The altered orientation of the c′′ strand in relation to the c′ and d strands directly affects the conformations of CDR2 and FR3 (Fig. 8). Therefore, the spatial integrity of the c′′ strand in relation to the c′ and d strands appears to impose a conformational restriction that is important for the binding motif of SEB and SEC3. This implies that other Vβ subsets that bind these SAGs may show c′′ conformational similarity to mouse Vβ8 and human Vβ12.
Several of the mutations that affected SEC3 binding appear to act by disrupting this conserved topology within the c′′ and d strand interactions. For example, 48βTyr interacts with 55βThr and 56βGlu on the c′′ strand. The side chain of 55βThr on the c′′ strand is hydrogen-bonded to its own main chain oxygen and involved in multiple vdw interactions with 69βArg on the d strand. This mutation could thereby disrupt hydrogen bonds that the c′ strand shares with SEC3 residues 20Thr and 23Asn.
The results shown here also provide a rationale for the importance of CDR2 residues 51–53 in the interactions of Vβ regions with SEC3 and SEB. Multiple hydrogen bonds and vdw interactions are formed with these Vβ8.2 residues and the conserved SAG residues 23Asn, 60Asn, and 90Tyr 16. However, the affinity of the Vβ8.2 region for SEB has been reported to be 100-fold less than for SEC3. Glyβ53 has 1 contact with SEB residue 23Asn but 11 additional vdw interactions with SEC3 residues 26Tyr and 210Gln. Since this position in CDR2 is a hotspot of SEC3 binding (ΔΔG > 2 kcal/mol), loss of these multiple contacts in the SEB complex may account for its lower affinity for the TCR compared with SEC3. Consistent with this possibility, mutation of SEB 26Val, which does not contact 53βGly, to the SEC3 residue tyrosine resulted in a higher affinity for the SEB/26Tyr variant (KD ∼ 12 μM; reference 17). Similarly, 51βGly has four contacts with SEC3 residue 91Val, but does not contact SEB residue 91Tyr.
Perhaps the most unexpected finding of the current study is that there were significant effects of many single-site mutations that are at the interface, but which lack direct side chain interactions with the wt SEC3 in the structure. In this regard, 57βLys is the only residue that exhibits side chain hydrogen bonds that contact SEC3. Other residues exhibit hydrogen bonds between main chain atoms and SEC3. One might expect these interactions to be largely sequence independent, consistent with the extensive diversity present among SEC3-reactive Vβ regions in the residues at the interface. With this in mind, it is reasonable to ask how do alanine substitutions reduce binding by up to 100-fold (e.g., Gly51βAla and Gly53βAla), if the primary interactions involve hydrogen bonds between SEC3 and Vβ main chain atoms? We consider two general features of a Vβ region that may be necessary (although not sufficient) for productive binding to SEC3. First, as previously discussed, the overall topology of the Vβ domain must conform to that observed in the crystal structures of the SEC3-reactive Vβ domains 12. Second, even with this scaffold, the conformations of CDR2 and HV4 loops can be affected by single residues, thereby impacting specific main chain interactions with SEC3. These effects on conformation might involve loop dynamics and mobility without altering the major backbone conformation observed in the crystal structures. A recent solution structure of an scTCR observed such mobility, but primarily in the CDR3 loops 50. Conformational plasticity was also observed in the interaction of the 2C TCR with the pMHC complex dEV8/Kb, but the major structural changes were in CDR3α and, to a lesser extent, CDR1α 51. In addition, studies of single-site variants in antigen–antibody interactions have shown that there can be entropic compensation for losses of enthalpy 52.
If single-site mutations observed in this report can so significantly affect SEC3 binding, then how do other SEC3-reactive Vβ regions, which have extensive diversity in this same set of residues (Table), maintain SEC3 reactivity? Although the answer to this question will come from detailed structural and mutagenic analyses of these Vβ regions, it is important to note that the binding affinities of SEC3 for most of the different Vβ regions have not been measured 12. It is possible that the variability found at key Vβ positions will confer upon these Vβ regions a range of affinities for SEC3, some of which differ significantly from that measured for the Vβ8.2 region. Thus, these interactions could exhibit a range of affinities that is as large as the difference between the affinities measured for Vβ8.2 interacting with SEB and with SEC3 (∼140-fold). From the functional perspective, this is not an unreasonable possibility, as it is clear that a 100-fold lower affinity of the TCR–SAG interaction may only affect the biological activity moderately, whereas the SAG–MHC affinity has a greater effect on function 17,53. As the Vα region is now thought to participate directly in the interaction with class II MHC (at least in some cases), it is also possible that some Vβ–SEC3 interactions are below the measurable affinity range, yet the entire VαVβ–SEC3–class II complexes are sufficiently stable to elicit T cell activity. In this model, the requirement for Vα–class II interaction (and perhaps CDR3 involvement) then depends on some basal affinity of the Vβ–SAG interaction. This possibility could be tested by measuring the affinities of the different Vβ regions for SEC3 and examining the restrictions in Vα use (focusing on CDR2α in particular) among T cells bearing the corresponding Vβ region. The latter approaches have already been taken for several SAGs, demonstrating that some appear to require either CDR3 or Vα interactions 8,19,20,21,22,23,24,25.
The observation that single amino acid differences in the area of contact can reduce the binding energy by >10-fold also provides an explanation for the diverse biological effects of the same SAG. Previous binding studies with TCR–pMHC have shown that there is as little as a threefold difference in affinities between agonist and antagonist responses 28,29. It is apparent that minor differences in Vβ sequence, or residues in CDR3β or the Vα region could either reduce or enhance the basal affinity of the Vβ–SAG–MHC interactions. The range of outcomes could be T cells that undergo full activation to T cells that undergo anergy.
We have also shown here that it is possible to increase the affinity of the TCR for SEC3 by approximately fivefold through the selected combination of alanine mutations. Although a fivefold increase is not likely to be sufficient for using soluble versions of the TCR as SEC3 antagonists, it does provide a guide for further engineering of high-affinity TCR by focusing on these residues. Furthermore, the findings suggest that other Vβ regions could have evolved compensatory mutations that act by increasing the affinities of the TCR for SAGs to some threshold level. A study of the human growth hormone receptor showed that analogous compensatory mutations at the hormone interface acted through local structural rearrangements in the region of contact 54. Some of these changes affected residues at 15 Å distance from the mutation. Likewise, the interactions of mutants of D5.1 antilysozyme and lysozyme have shown that altered residues can compensate, even at long distances, through reorganization of solvent at the interface 52,55. It is reasonable to think that the other Vβ regions will likewise have local structural rearrangements at the SEC3 interface in order to achieve the minimal affinity necessary (KD ∼100 μM) for stimulating T cells. The fact that multiple solutions to this interaction might have evolved among Vβ regions with the same scaffold suggests that there was strong selective pressure on SAGs to maintain binding to these diverse T cells.
We thank Drs. T. Brodnicki, T. Manning, and C. Schlueter for their contributions in scTCR mutagenesis, and N. Bivens and the W. M. Keck Center for Comparative and Functional Genomics at the University of Illinois for oligonucleotide synthesis and DNA sequencing.
This work was supported by National Institutes of Health grant GM55767 (to D.M. Kranz), AI36900 and AI42937 (to R.A. Mariuzza), and National Multiple Sclerosis Society grant RG2747 (to R.A. Mariuzza). P.S. Andersen is a Fellow of the Danish Natural Science Research Council.
Abbreviations used in this paper: ΔΔG, change in free energy; FR, framework region; HRP, horseradish peroxidase; HV, hypervariable region; IC50, half-maximal inhibitory concentration; KD, equilibrium binding constant; pMHC, peptide-MHC complex; SAG, superantigen; sc, single-chain; SE, Staphylococcus aureus enterotoxin; SPR, surface plasmon resonance; vdw, Van der Waals; wt, wild-type.