After artificial cultivation for a period of over 3 years Bacterium pneumosintes has maintained its original morphological and cultural characteristics, when grown in the original medium. Adaptation to a saprophytic existence has been accompanied by a loss of pathogenicity. Our strains now grow readily under strictly anaerobic conditions in a variety of media with peptone broth as a base, enriched with fresh tissue, blood, or by the growth of other bacteria. Surface colonies have been obtained on blood agar plates in. an anaerobic jar. These various methods of cultivation are adapted to special purposes. In broth cultures Bacterium pneumosintes grows in larger forms than in the ascitic fluid-tissue medium, but the identity of the microorganisms is proved by their serological reactions and by reversion to the minute forms on transfer to the original medium.
Article|
June 01 1922
EXPERIMENTAL STUDIES OF THE NASOPHARYNGEAL SECRETIONS FROM INFLUENZA PATIENTS : VII. FURTHER OBSERVATIONS ON THE CULTURAL AND MORPHOLOGICAL CHARACTERS OF BACTERIUM PNEUMOSINTES.
Peter K. Olitsky,
Peter K. Olitsky
From the Laboratories of The Rockefeller Institute for Medical Research.
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Frederick L. Gates
Frederick L. Gates
From the Laboratories of The Rockefeller Institute for Medical Research.
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Peter K. Olitsky
From the Laboratories of The Rockefeller Institute for Medical Research.
Frederick L. Gates
From the Laboratories of The Rockefeller Institute for Medical Research.
Received:
February 12 1922
Online ISSN: 1540-9538
Print ISSN: 0022-1007
Copyright, 1922, by The Rockefeller Institute for Medical Research New York
1922
J Exp Med (1922) 35 (6): 813–821.
Article history
Received:
February 12 1922
Citation
Peter K. Olitsky, Frederick L. Gates; EXPERIMENTAL STUDIES OF THE NASOPHARYNGEAL SECRETIONS FROM INFLUENZA PATIENTS : VII. FURTHER OBSERVATIONS ON THE CULTURAL AND MORPHOLOGICAL CHARACTERS OF BACTERIUM PNEUMOSINTES. . J Exp Med 1 June 1922; 35 (6): 813–821. doi: https://doi.org/10.1084/jem.35.6.813
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