Results of experiments on the preparation of high molecular weight constituents from normal and poliomyelitis-infected medullae-cords are presented. Relatively large yields were obtained from glycerolated normal or infected tissues or from tissues that had been stored at –10°C. for a few days. When the frozen tissues were stored for several weeks, the amount of sedimentable nitrogen isolated from the normal cords decreased and became almost negligible. Under these same conditions small but definite amounts of a high molecular weight material were isolated from the infectious extracts. This material regularly produced poliomyelitis when 1 ml. containing 5 x 10–9 gm. of nitrogen was injected intracerebrally into rhesus monkeys. The purified virus sediment contains nitrogen, phosphorus, and carbohydrate, it gives the Millon's test for protein, and is precipitated by one-third saturation with ammonium sulfate. Results of ultracentrifugal analyses show that a relatively homogeneous component with SW20° = 62 x 10–13 is present.
Article|
April 01 1942
STUDIES ON THE PURIFICATION OF POLIOMYELITIS VIRUS : I. YIELDS AND ACTIVITY OF PREPARATIONS OBTAINED BY DIFFERENTIAL CENTRIFUGATION
Hubert S. Loring,
Hubert S. Loring
From the Department of Chemistry, Stanford University, California
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C. E. Schwerdt
C. E. Schwerdt
From the Department of Chemistry, Stanford University, California
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Hubert S. Loring
From the Department of Chemistry, Stanford University, California
C. E. Schwerdt
From the Department of Chemistry, Stanford University, California
Received:
December 28 1941
Online ISSN: 1540-9538
Print ISSN: 0022-1007
Copyright, 1942, by The Rockefeller Institute for Medical Research New York
1942
J Exp Med (1942) 75 (4): 395–406.
Article history
Received:
December 28 1941
Citation
Hubert S. Loring, C. E. Schwerdt; STUDIES ON THE PURIFICATION OF POLIOMYELITIS VIRUS : I. YIELDS AND ACTIVITY OF PREPARATIONS OBTAINED BY DIFFERENTIAL CENTRIFUGATION . J Exp Med 1 April 1942; 75 (4): 395–406. doi: https://doi.org/10.1084/jem.75.4.395
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