Films of several proteins, hen ovalbumin, horse and human serum albumins, and globulins were found to combine specifically with antibodies, showing that the reactions can take place independently of the structure which secures the compact shape of the protein molecule. Serum globulin films differed from ovalbumin in that they lost their reactivity when kept on the water surface. Species specific reactions were observed with films of serum albumin and suitably diluted antisera, and likewise in the customary precipitin tests in which immune sera for denatured hen ovalbumin were tested against ovalbumin of other species. Specific fixation was also observed upon exposing monolayers of purified antibodies for pneumococci of Types I and III to the corresponding polysaccharides.
Article|
November 01 1942
SEROLOGICAL REACTIONS OF PROTEIN FILMS AND DENATURED PROTEINS
A. Rothen,
A. Rothen
From the Laboratories of The Rockefeller Institute for Medical Research
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K. Landsteiner
K. Landsteiner
From the Laboratories of The Rockefeller Institute for Medical Research
Search for other works by this author on:
A. Rothen
From the Laboratories of The Rockefeller Institute for Medical Research
K. Landsteiner
From the Laboratories of The Rockefeller Institute for Medical Research
Received:
June 24 1942
Online ISSN: 1540-9538
Print ISSN: 0022-1007
Copyright, 1942, by The Rockefeller Institute for Medical Research New York
1942
J Exp Med (1942) 76 (5): 437–450.
Article history
Received:
June 24 1942
Citation
A. Rothen, K. Landsteiner; SEROLOGICAL REACTIONS OF PROTEIN FILMS AND DENATURED PROTEINS . J Exp Med 1 November 1942; 76 (5): 437–450. doi: https://doi.org/10.1084/jem.76.5.437
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