Nitella cells were exposed to various oxidants and reductants, to determine their effect upon the bioelectric potential. These included five systems, with an Eh range from +0.454 v. to –0.288 v., a total range of 0.742 v.
When proper regard was given to buffering against acidity changes, and concentration changes of Na or K ions in the oxidized and reduced forms, no significant effect upon the bioelectric potential was found:
1. When an oxidant or reductant (K ferri- or ferrocyanide) was applied instead of an equivalent normality of an "indifferent" salt (KCl).
2. In changing from a given oxidant to its corresponding reductant (ferri- to ferrocyanide; oxidized to leuco-dye, etc.).
3. When a mixture of 2 dyes, (indophenol with positive E'0, and safranin with negative E'0) was oxidized and reduced, to give better poising at the extremes.
It is conduded that the outer surface of this cell is not influenced by the state of oxidation or reduction of the systems employed; at least it does not respond with a manifest change of bioelectric potential to changes in oxidation-reduction intensity of the medium.
The cells continued to show, however, at all times their usual response to concentration changes of KCl, NaCl, etc., and to electrical stimulation.