The problem of the resolution and reconstitution of the inner mitochondrial membrane has been approached at three levels. (1) Starting with phosphorylating submitochondrial particles, a "resolution from without" can be achieved by stripping of surface components. The most extensive resolution was recently obtained with the aid of silicotungstate. Such particles require for oxidative phosphorylation the addition of several coupling factors as well as succinate dehydrogenase. (2) Starting with submitochondrial particles that have been degraded by trypsin and urea a resolution of the inner membrane proper containing an ATPase has been achieved. These experiments show that at least five components are required for the reconstitution of an oligomycin-sensitive ATPase: a particulate component, F1, Mg++, phospholipids, and Fc. Morphologically, the reconstituted ATPase preparations resemble submitochondrial particles. (3) Starting with intact mitochondria individual components of the oxidation chain have been separated from each other. The following components were required for the reconstitution of succinoxidase: succinate dehydrogenase, cytochrome b\, cytochrome c1, cytochrome c, cytochrome oxidase, phospholipids and Q10. The reconstituted complex had properties similar to those of phosphorylating submitochondrial particles; i.e., the oxidation of succinate by molecular oxygen was highly sensitive to antimycin.
Article| July 01 1969
Resolution and Reconstitution of a Mammalian Membrane
From the Section of Biochemistry and Molecular Biology, Cornell University, Ithaca, New York 14850
Online ISSN: 1540-7748
Print ISSN: 0022-1295
Copyright © 1969 by The Rockefeller University Press
J Gen Physiol (1969) 54 (1): 38–49.
Efraim Racker; Resolution and Reconstitution of a Mammalian Membrane . J Gen Physiol 1 July 1969; 54 (1): 38–49. doi: https://doi.org/10.1085/jgp.54.1.38
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