The transport of some sugars at the antiluminal face of renal cells was studied using teased tubules of flounder (Pseudopleuronectes americanus). The analytical procedure allowed the determination of both free and total (free plus phosphorylated) tissue sugars. The inulin space of the preparation was 0.333 ± 0.017 kg/kg wet wt (7 animals, 33 analyses). The nonmetabolizable α-methyl-D-glucoside entered the cells by a carrier-mediated (phloridzin-sensitive), ouabain-insensitive process. The steady-state tissue/medium ratio was systematically below that for diffusion equilibrium. D-Glucose was a poor inhibitor of α-methyl-glucoside transport, D-galactose was ineffective. The phloridzin-sensitive transport processes of 2-deoxy-D-glucose,D-galactose,and 2-deoxy-D-galactose were associated with considerable phosphorylation. Kinetic evidence suggested that these sugars were transported in free form and subsequently were phosphorylated. 2-Deoxy-D-glucose accumulated in the cells against a slight concentration gradient. This transport was greatly inhibited by D-glucose, whereas α-methyl-glucoside and also D-galactose and its 2-deoxy-derivative were ineffective. D-Galactose and 2-deoxy-D-galactose mutually competed for transport; D-glucose, 2-deoxy-D-glucose, and α-methyl-D-glucoside were ineffective. Studies using various sugars as inhibitors suggest the presence of three carrier-mediated pathways of sugar transport at the antiluminal cell face of the flounder renal tubule: the pathway of α-methyl-D-glucoside (not shared by D-glucose); the pathway commonly shared by 2-deoxy-D-glucose and D-glucose; the pathway shared by D-galactose and 2-deoxy-D-galactose.
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August 01 1973
Sugar Transport across the Peritubular Face of Renal Cells of the Flounder
Arnost Kleinzeller,
Arnost Kleinzeller
From the Department of Physiology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19174, and the Mount Desert Island Biological Laboratory, Salsbury Cove, Maine 04672
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Elizabeth M. McAvoy
Elizabeth M. McAvoy
From the Department of Physiology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19174, and the Mount Desert Island Biological Laboratory, Salsbury Cove, Maine 04672
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Arnost Kleinzeller
From the Department of Physiology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19174, and the Mount Desert Island Biological Laboratory, Salsbury Cove, Maine 04672
Elizabeth M. McAvoy
From the Department of Physiology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19174, and the Mount Desert Island Biological Laboratory, Salsbury Cove, Maine 04672
Received:
February 27 1973
Online ISSN: 1540-7748
Print ISSN: 0022-1295
Copyright © 1973 by The Rockefeller University Press
1973
J Gen Physiol (1973) 62 (2): 169–184.
Article history
Received:
February 27 1973
Citation
Arnost Kleinzeller, Elizabeth M. McAvoy; Sugar Transport across the Peritubular Face of Renal Cells of the Flounder . J Gen Physiol 1 August 1973; 62 (2): 169–184. doi: https://doi.org/10.1085/jgp.62.2.169
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