To determine whether a cell metabolite was involved in active calcium transport, the cell contents of human erythrocytes were subjected to high dilutions and the resultant ghosts were checked for their ability to actively transport calcium. It was found that the diluted erythrocyte ghosts did retain their capacity to actively transport calcium and that the characteristics of this transport process appeared to be unaltered by the high dilutions. Calcium analysis of the cell membrane and cell supernatant indicated that almost all of the calcium was lost from the cell solution rather than the cell membrane as active calcium transport proceeded. Therefore it appeared that calcium was able to cross the cell membrane without the aid of a cell metabolite. Investigations with layered erythrocytes indicated that the active transport of calcium was not assisted by centrifugation. Neither inorganic phosphate, pyrophosphate, nor an adenine nucleotide appeared to accompany calcium across the membrane as indicated by total phosphate and inorganic phosphate analysis and 260-nm readings of the deproteinized supernatant.
Article|
May 01 1974
Investigation of the Accompaniment of Calcium During Active Calcium Transport from Human Erythrocyte Ghosts
Erik J. Olson,
Erik J. Olson
From the Department of Pharmacology, Meharry Medical College, Nashville, Tennessee 37208
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Ralph J. Cazort
Ralph J. Cazort
From the Department of Pharmacology, Meharry Medical College, Nashville, Tennessee 37208
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Erik J. Olson
From the Department of Pharmacology, Meharry Medical College, Nashville, Tennessee 37208
Ralph J. Cazort
From the Department of Pharmacology, Meharry Medical College, Nashville, Tennessee 37208
Received:
October 02 1973
Online ISSN: 1540-7748
Print ISSN: 0022-1295
1974
J Gen Physiol (1974) 63 (5): 590–600.
Article history
Received:
October 02 1973
Citation
Erik J. Olson, Ralph J. Cazort; Investigation of the Accompaniment of Calcium During Active Calcium Transport from Human Erythrocyte Ghosts . J Gen Physiol 1 May 1974; 63 (5): 590–600. doi: https://doi.org/10.1085/jgp.63.5.590
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