Issues

Cover picture: Pixel-by-pixel Förster resonance energy transfer (FRET) amplitude distribution profiles from cells expressing fluorescent GAT1 GABA transporters correlate with transporter oligomerization states and function. (Top) Normalized FRET (NFRET) between coexpressed CFP- and YFP-tagged GAT1 constructs is analyzed by region of interest (ROI). Bars, 10 μm. (Bottom) GAT1 dimers, high-order oligomers, and transporters forming PDZ-mediated interactions with the cytoskeleton and/or the exocyst are resolved as distinct Gaussian components of the NFRET distributions (see tutorial research article by Moss et al., 489–521).