Table 1.
Sequences with the changes introduced in the different mutants
MutantSequence (5′–3′)
WT GGT​GAT​GAC​CCG​TAG​ACG​ATT​CTA​TCC​GGG​GGC​TGC​CAA​TGA​GCA​GA 
pydZU5-2 GGT​GAT​GAC​CCG​TAG​ACG​AT–––––CCG​GGG​GCT​GCC​AAT​GAG​CAG​A 
WT CGA​TTT​CCT​ATT​CCC​CAT​GAC​CAC​AT––CCC​GAC​TCT​CGT 
pydα-9 CGA​TTT​CCT​ATT​CCC​CAT​GAC​CAC​ATG​ACC​CGA​CTC​TCG​T 
WT CGA​GTG​GAG​TGT​ACC​AAA​TCG​C––––GGA​TAG​GGT​CTA​TAC​CAC​CAG 
pydCC6 CGA​GTG​GAG​TGT​ACC​AAA​TAG​GAT​AGG​GAT​AGG​GTC​TAT​ACC​ACC​AG 
WT CGC​TAA​TAC​TGG​CAC​CAT​ATC​CTC​TAC​GAA​TGG​CTA​CGG​ATC​GCT​G 
pydS-10 CGC​TAA​TAC​TGG​CAC​CAT​ATC​CTC––––––––––TAC​GGA​TCG​CTG 
MutantSequence (5′–3′)
WT GGT​GAT​GAC​CCG​TAG​ACG​ATT​CTA​TCC​GGG​GGC​TGC​CAA​TGA​GCA​GA 
pydZU5-2 GGT​GAT​GAC​CCG​TAG​ACG​AT–––––CCG​GGG​GCT​GCC​AAT​GAG​CAG​A 
WT CGA​TTT​CCT​ATT​CCC​CAT​GAC​CAC​AT––CCC​GAC​TCT​CGT 
pydα-9 CGA​TTT​CCT​ATT​CCC​CAT​GAC​CAC​ATG​ACC​CGA​CTC​TCG​T 
WT CGA​GTG​GAG​TGT​ACC​AAA​TCG​C––––GGA​TAG​GGT​CTA​TAC​CAC​CAG 
pydCC6 CGA​GTG​GAG​TGT​ACC​AAA​TAG​GAT​AGG​GAT​AGG​GTC​TAT​ACC​ACC​AG 
WT CGC​TAA​TAC​TGG​CAC​CAT​ATC​CTC​TAC​GAA​TGG​CTA​CGG​ATC​GCT​G 
pydS-10 CGC​TAA​TAC​TGG​CAC​CAT​ATC​CTC––––––––––TAC​GGA​TCG​CTG 

WT from FlyBase release FB2019_01.

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