Rockefeller University Press

Table 1

Summary of γδ CTL Clones

Clone^*	Vγ usage (RT-PCR)	γδTCR expression^§			Surface antigens (FACS^®)			Blocking of the cytotoxicity with antibody^¶
Clone^*	Vγ usage (RT-PCR)	TCRγδ	TCRδ	Vγ4	CD4/CD8^‖	Thy-1	Ly-1	TL	H-2	TCRδ≳≳	Lyt-2	Lyt-3	L3T4
KC1-3	1/2^‡	+	+	−	CD8αβ	+	+	+	−	+	+	+	−
1-7	4^‡	+	+	+	CD8αβ	+	+	+	−	+	+	+	−
4-4	2/4^‡	+	+	+	CD8αβ	+	+	+	−	+	+	+	−
4-8	1/2/4^‡	+	+	+	DN	+	+	+	−	+	−	−	−
4-9	2/4	+	+	+	CD8αβ	+	+	+	−	+	+	+	−
5-1	2/4	+	+	+	CD8αβ	+	+	+	−	+	+	+	−
5-2	2/4	+	+	+	CD8αβ	+	+	+	−	+	+	+	−
6-1	2/4^‡	+	+	+	DN	+	+	+	−	+	−	−	−
6-2	2/4	+	+	+	CD8αβ	+	+	+	−	+	+	+	−
7-3	1/2/4	+	+	+	CD8αβ	+	+	+	−	+	+	+	−
7-4	1/2^‡	+	+	−	DN	+	+	+	−	+	−	−	−
8-1	2/4	+	+	+	DN	+	+	+	−	+	−	−	−
8-3	2/4^‡	+	+	+	DN	+	+	+	−	+	−	−	−
8-4	2/4	+	+	+	DN	+	+	+	−	+	−	−	−

Clone^*	Vγ usage (RT-PCR)	γδTCR expression^§			Surface antigens (FACS^®)			Blocking of the cytotoxicity with antibody^¶
Clone^*	Vγ usage (RT-PCR)	TCRγδ	TCRδ	Vγ4	CD4/CD8^‖	Thy-1	Ly-1	TL	H-2	TCRδ≳≳	Lyt-2	Lyt-3	L3T4
KC1-3	1/2^‡	+	+	−	CD8αβ	+	+	+	−	+	+	+	−
1-7	4^‡	+	+	+	CD8αβ	+	+	+	−	+	+	+	−
4-4	2/4^‡	+	+	+	CD8αβ	+	+	+	−	+	+	+	−
4-8	1/2/4^‡	+	+	+	DN	+	+	+	−	+	−	−	−
4-9	2/4	+	+	+	CD8αβ	+	+	+	−	+	+	+	−
5-1	2/4	+	+	+	CD8αβ	+	+	+	−	+	+	+	−
5-2	2/4	+	+	+	CD8αβ	+	+	+	−	+	+	+	−
6-1	2/4^‡	+	+	+	DN	+	+	+	−	+	−	−	−
6-2	2/4	+	+	+	CD8αβ	+	+	+	−	+	+	+	−
7-3	1/2/4	+	+	+	CD8αβ	+	+	+	−	+	+	+	−
7-4	1/2^‡	+	+	−	DN	+	+	+	−	+	−	−	−
8-1	2/4	+	+	+	DN	+	+	+	−	+	−	−	−
8-3	2/4^‡	+	+	+	DN	+	+	+	−	+	−	−	−
8-4	2/4	+	+	+	DN	+	+	+	−	+	−	−	−

The first digit of the clone name shows the origin of the mouse.

RT-PCR products were sequenced (see Fig. 6).

Expression of TCRγδ, TCRδ, and Vγ4 chains on the cell surface were detected by FACS^® using moAb GL3, 3A10, and UC3-10A6, respectively.

Monoclonal antibodies to L3T4, Lyt-2 and Lyt-3 were used.

The target cells were Con A blasts of spleen cells from Tg.Con.3-1 (T3^b-TL positive).

Monoclonal antibody 3A10 was used.