Leukocytes in Epidermal Cell Suspensions from TGF-β1 Null and Control Mice
| Experiment . | . | Age . | . | Genotype . | . | LC (%)* . | . | DETC (%)* . | . | Leukocytes (%)* . |
|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 8d | −/− | 0.2 | ND | 2.5 | |||||
| +/+ | 5.5 | ND | 6.5 | |||||||
| 2 | 10d | −/− | 0.2 | 2.7 (3.2) | 3.6 | |||||
| +/+ | 5.4 | 3.8 (4.2) | 8.5 | |||||||
| 3 | 18d | −/− | 0 | 4.9 | 4.8 | |||||
| +/− | 3.8 | 3.5 | 6.3 | |||||||
| +/+ | 4.5 | 4.4 | 10.5 | |||||||
| 4 | 17d | −/− | 0.1 | ND | 6.7 | |||||
| +/− | 6.1 | ND | 13.0 | |||||||
| +/+ | 6.5 | ND | 11.9 | |||||||
| 5 | 14d | −/− | 0.1 | ND | 6.7 | |||||
| +/− | 5.4 | ND | 10.6 | |||||||
| +/+ | 8.9 | ND | 13.7 | |||||||
| 6 | 17d | −/− | 0 | 7.6 | 7.6 | |||||
| +/+ | 5.2 | 6.6 | 12.4 | |||||||
| 7 | 14d | −/− | 0 | ND | 6.9 | |||||
| +/+ | 3.0 | ND | 6.5 | |||||||
| Summary** | ||||||||||
| −/− | 0.1 ± 0.1 (n = 7) | 5.1 ± 2.0 (n = 3) | 5.5 ± 1.8 (n = 7) | |||||||
| +/− | 5.1 ± 1.0 (n = 3) | 3.5 (n = 1) | 10.0 ± 2.8 (n = 3) | |||||||
| +/+ | 5.6 ± 1.7 (n = 7) | 4.9 ± 1.2 (n = 3) | 10.0 ± 2.7 (n = 7) |
| Experiment . | . | Age . | . | Genotype . | . | LC (%)* . | . | DETC (%)* . | . | Leukocytes (%)* . |
|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 8d | −/− | 0.2 | ND | 2.5 | |||||
| +/+ | 5.5 | ND | 6.5 | |||||||
| 2 | 10d | −/− | 0.2 | 2.7 (3.2) | 3.6 | |||||
| +/+ | 5.4 | 3.8 (4.2) | 8.5 | |||||||
| 3 | 18d | −/− | 0 | 4.9 | 4.8 | |||||
| +/− | 3.8 | 3.5 | 6.3 | |||||||
| +/+ | 4.5 | 4.4 | 10.5 | |||||||
| 4 | 17d | −/− | 0.1 | ND | 6.7 | |||||
| +/− | 6.1 | ND | 13.0 | |||||||
| +/+ | 6.5 | ND | 11.9 | |||||||
| 5 | 14d | −/− | 0.1 | ND | 6.7 | |||||
| +/− | 5.4 | ND | 10.6 | |||||||
| +/+ | 8.9 | ND | 13.7 | |||||||
| 6 | 17d | −/− | 0 | 7.6 | 7.6 | |||||
| +/+ | 5.2 | 6.6 | 12.4 | |||||||
| 7 | 14d | −/− | 0 | ND | 6.9 | |||||
| +/+ | 3.0 | ND | 6.5 | |||||||
| Summary** | ||||||||||
| −/− | 0.1 ± 0.1 (n = 7) | 5.1 ± 2.0 (n = 3) | 5.5 ± 1.8 (n = 7) | |||||||
| +/− | 5.1 ± 1.0 (n = 3) | 3.5 (n = 1) | 10.0 ± 2.8 (n = 3) | |||||||
| +/+ | 5.6 ± 1.7 (n = 7) | 4.9 ± 1.2 (n = 3) | 10.0 ± 2.7 (n = 7) |
Epidermal cell suspensions were prepared from TGF-β1 −/− mice and littermate controls and stained for CD45 and I-A antigens (LC), CD45 and TCRγδ (DETC), and CD45 and isotype control (Leukocytes), and analyzed using multicolor flow cytometry. In some experiments, DETC were also identified as CD45+Thy-1.2+ cells (% in parentheses). Nonviable cells were excluded by live gating. Data are presented as percentages of events accumulated and represent individual determinations (*) or the mean ± SEM (**) of individual determinations.