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Table 1

D-Lc Can Drive B Cells to Secrete Both IgA1 and IgA2 in Response to Cytokines

ExperimentIgA1 (ng/ml)IgA2 (ng/ml)
Without D-LcWith D-LcWithout D-LcWith D-Lc
 IL-10    413 ± 350    2456 ± 1116   58 ± 20 
  IL-10 + TGFβ    725 ± 283  6051 ± 812   5 ± 2   180 ± 69 
 IL-10  1125 ± 117  6339 ± 432   6 ± 2   169 ± 14 
  IL-10 + TGFβ  2091 ± 215    8429 ± 1113  12 ± 4   307 ± 41 
 IL-10  181 ± 84  9492 ± 966   583 ± 174 
  IL-10 + TGFβ    507 ± 344  15816 ± 3844   806 ± 287 
ExperimentIgA1 (ng/ml)IgA2 (ng/ml)
Without D-LcWith D-LcWithout D-LcWith D-Lc
 IL-10    413 ± 350    2456 ± 1116   58 ± 20 
  IL-10 + TGFβ    725 ± 283  6051 ± 812   5 ± 2   180 ± 69 
 IL-10  1125 ± 117  6339 ± 432   6 ± 2   169 ± 14 
  IL-10 + TGFβ  2091 ± 215    8429 ± 1113  12 ± 4   307 ± 41 
 IL-10  181 ± 84  9492 ± 966   583 ± 174 
  IL-10 + TGFβ    507 ± 344  15816 ± 3844   806 ± 287 

5 × 104/ml slgD+ B lymphocytes were cultured for 14 d in a final volume of 200 μl in the presence or absence of 5 × 104/ml D-Lc over 1.25 × 104/ml irradiated CD40L-L cells with IL-10 (200 ng/ml) or with IL-10 + TGFβ (1 ng/ml). IgA1 and IgA2 levels in culture supernatants were measured in specific ELISAs as described in Materials and Methods. Results are expressed as mean ± SD of triplicate cultures.  

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