D-Lc Can Drive B Cells to Secrete Both IgA1 and IgA2 in Response to Cytokines
| Experiment . | . | . | . | IgA1 (ng/ml) . | . | IgA2 (ng/ml) . | ||||
|---|---|---|---|---|---|---|---|---|---|---|
| Without D-Lc . | . | With D-Lc . | Without D-Lc . | . | With D-Lc . | |||||
| 1 | IL-10 | 413 ± 350 | 2456 ± 1116 | 0 | 58 ± 20 | |||||
| IL-10 + TGFβ | 725 ± 283 | 6051 ± 812 | 5 ± 2 | 180 ± 69 | ||||||
| 2 | IL-10 | 1125 ± 117 | 6339 ± 432 | 6 ± 2 | 169 ± 14 | |||||
| IL-10 + TGFβ | 2091 ± 215 | 8429 ± 1113 | 12 ± 4 | 307 ± 41 | ||||||
| 3 | IL-10 | 181 ± 84 | 9492 ± 966 | 0 | 583 ± 174 | |||||
| IL-10 + TGFβ | 507 ± 344 | 15816 ± 3844 | 0 | 806 ± 287 | ||||||
| Experiment . | . | . | . | IgA1 (ng/ml) . | . | IgA2 (ng/ml) . | ||||
|---|---|---|---|---|---|---|---|---|---|---|
| Without D-Lc . | . | With D-Lc . | Without D-Lc . | . | With D-Lc . | |||||
| 1 | IL-10 | 413 ± 350 | 2456 ± 1116 | 0 | 58 ± 20 | |||||
| IL-10 + TGFβ | 725 ± 283 | 6051 ± 812 | 5 ± 2 | 180 ± 69 | ||||||
| 2 | IL-10 | 1125 ± 117 | 6339 ± 432 | 6 ± 2 | 169 ± 14 | |||||
| IL-10 + TGFβ | 2091 ± 215 | 8429 ± 1113 | 12 ± 4 | 307 ± 41 | ||||||
| 3 | IL-10 | 181 ± 84 | 9492 ± 966 | 0 | 583 ± 174 | |||||
| IL-10 + TGFβ | 507 ± 344 | 15816 ± 3844 | 0 | 806 ± 287 | ||||||
5 × 104/ml slgD+ B lymphocytes were cultured for 14 d in a final volume of 200 μl in the presence or absence of 5 × 104/ml D-Lc over 1.25 × 104/ml irradiated CD40L-L cells with IL-10 (200 ng/ml) or with IL-10 + TGFβ (1 ng/ml). IgA1 and IgA2 levels in culture supernatants were measured in specific ELISAs as described in Materials and Methods. Results are expressed as mean ± SD of triplicate cultures.