Comparative Phenotype of CD40 versus GM–CSF/ TNF-derived DC
| . | . | CD40–DC . | . | GM–CSF/TNF–DC . |
|---|---|---|---|---|
| CD1a | − | ++ | ||
| CD2 | − | − | ||
| CD4 | ± (sb) | ++ | ||
| CD14 | − | − | ||
| CD15 | − | − | ||
| CD16 | − | − | ||
| CD19 | − | − | ||
| CD20 | − | − | ||
| CD21 | − | − | ||
| CD23 | − | − | ||
| CD26 | +++ | ND | ||
| CD32 | − | ++ | ||
| CD34 | − | − | ||
| CD40 | − | ++ | ||
| CD43 | +++ | ++ | ||
| CD44 | − | ++ | ||
| CD45 | +++ | ++ | ||
| CD59 | − | ++ | ||
| CD68 | − | + (sb) | ||
| CD74 | ++ | ++ | ||
| CD80 | + | ++ | ||
| CD86 | ++ | ++ | ||
| CD90 | − | − | ||
| Pan-Cadherin | + | ND | ||
| NGFR | +++ | − | ||
| HSP60 | ++ | ND | ||
| MHC class II | +++ | +++ | ||
| MHC class I | +++ | +++ | ||
| rel-B | +++ | + | ||
| Phenotype of CD40-derived DC, comparison to GM–CSF/TNFderived DC. 104 DC harvested at day 14 were deposited onto BioRad slides to perform immunostaining. Culture conditions, preparation of cells, and staining procedures as described in Fig. 2. For intracytoplasmic staining, saponin at 0.33% in PBS was used throughout the staining protocol. Cells were scored as positive when a clearer and stronger staining than that of control antibodies was observed. Reactivity with the different antibodies tested was scored as (−) negative, (±) weak, (+) modest, (++) intermediate, to strong (+++), based on staining intensity. In addition, (sb) indicates reactivity only with a subset (<50%) of the DC. | ||||
| . | . | CD40–DC . | . | GM–CSF/TNF–DC . |
|---|---|---|---|---|
| CD1a | − | ++ | ||
| CD2 | − | − | ||
| CD4 | ± (sb) | ++ | ||
| CD14 | − | − | ||
| CD15 | − | − | ||
| CD16 | − | − | ||
| CD19 | − | − | ||
| CD20 | − | − | ||
| CD21 | − | − | ||
| CD23 | − | − | ||
| CD26 | +++ | ND | ||
| CD32 | − | ++ | ||
| CD34 | − | − | ||
| CD40 | − | ++ | ||
| CD43 | +++ | ++ | ||
| CD44 | − | ++ | ||
| CD45 | +++ | ++ | ||
| CD59 | − | ++ | ||
| CD68 | − | + (sb) | ||
| CD74 | ++ | ++ | ||
| CD80 | + | ++ | ||
| CD86 | ++ | ++ | ||
| CD90 | − | − | ||
| Pan-Cadherin | + | ND | ||
| NGFR | +++ | − | ||
| HSP60 | ++ | ND | ||
| MHC class II | +++ | +++ | ||
| MHC class I | +++ | +++ | ||
| rel-B | +++ | + | ||
| Phenotype of CD40-derived DC, comparison to GM–CSF/TNFderived DC. 104 DC harvested at day 14 were deposited onto BioRad slides to perform immunostaining. Culture conditions, preparation of cells, and staining procedures as described in Fig. 2. For intracytoplasmic staining, saponin at 0.33% in PBS was used throughout the staining protocol. Cells were scored as positive when a clearer and stronger staining than that of control antibodies was observed. Reactivity with the different antibodies tested was scored as (−) negative, (±) weak, (+) modest, (++) intermediate, to strong (+++), based on staining intensity. In addition, (sb) indicates reactivity only with a subset (<50%) of the DC. | ||||