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Table 2

INF-γ–deficient RIP LCMV tg Mice Have Fewer LCMV-specific Memory CTLs in the Pancreas than their IFN-γ–competent Littermates

Precursor frequency of CTLKilling of H-2d targets
Secondary effector day 60 CTLH-2dH-2b
GroupOriginLCMVvv/GPNPpepLCMVLCMV
H-2d IFN-γ–competent  Spleen  1/1,500  NDT  1/2,500  NDT  60 ± 7% 
H-2d IFN-γ–deficient  Spleen  1/3,000  NDT  1/4,200  NDT  54 ± 9% 
RIP NP, H-2d IFN-γ–competent  Spleen  1/3,000  1/9,000  1/3,900  NDT  34 ± 8% 
RIP NP, H-2d IFN-γ–deficient  Spleen  1/5,000  1/9,000  1/7,000  NDT  35 ± 2% 
RIP NP, IDDM IFN-γ–competent  Pancreas  1/2,000  not done  1/6,000  NDT  38 ± 7% 
RIP NP, no IDDM γ-IFN–deficient  Pancreas  1/20,000  NDT  NDT  NDT  18 ± 5% 
Secondary CTLs were recovered from spleens and pancreata of infected (103 PFU LCMV intraperitoneally) IFN-γ–deficient RIP LCMV mice, single transgenic RIP LCMV mice, and nontransgenic IFN-γ–deficient or –competent controls. CTL activity was tested on syngeneic LCMV infected or uninfected target cells after a 5–14 d in vitro stimulation (see Materials and Methods), and precursor frequency analysis was performed as described (26). NDT, not detectable (<1/60,000). 
Precursor frequency of CTLKilling of H-2d targets
Secondary effector day 60 CTLH-2dH-2b
GroupOriginLCMVvv/GPNPpepLCMVLCMV
H-2d IFN-γ–competent  Spleen  1/1,500  NDT  1/2,500  NDT  60 ± 7% 
H-2d IFN-γ–deficient  Spleen  1/3,000  NDT  1/4,200  NDT  54 ± 9% 
RIP NP, H-2d IFN-γ–competent  Spleen  1/3,000  1/9,000  1/3,900  NDT  34 ± 8% 
RIP NP, H-2d IFN-γ–deficient  Spleen  1/5,000  1/9,000  1/7,000  NDT  35 ± 2% 
RIP NP, IDDM IFN-γ–competent  Pancreas  1/2,000  not done  1/6,000  NDT  38 ± 7% 
RIP NP, no IDDM γ-IFN–deficient  Pancreas  1/20,000  NDT  NDT  NDT  18 ± 5% 
Secondary CTLs were recovered from spleens and pancreata of infected (103 PFU LCMV intraperitoneally) IFN-γ–deficient RIP LCMV mice, single transgenic RIP LCMV mice, and nontransgenic IFN-γ–deficient or –competent controls. CTL activity was tested on syngeneic LCMV infected or uninfected target cells after a 5–14 d in vitro stimulation (see Materials and Methods), and precursor frequency analysis was performed as described (26). NDT, not detectable (<1/60,000). 
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