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Table 2

INF-γ–deficient RIP LCMV tg Mice Have Fewer LCMV-specific Memory CTLs in the Pancreas than their IFN-γ–competent Littermates

		Precursor frequency of CTL				Killing of H-2^d targets
Secondary effector day 60 CTL		H-2^d			H-2^b	Killing of H-2^d targets
Group	Origin	LCMV	vv/GP	NPpep	LCMV	LCMV
H-2^d IFN-γ–competent	Spleen	1/1,500	NDT	1/2,500	NDT	60 ± 7%
H-2^d IFN-γ–deficient	Spleen	1/3,000	NDT	1/4,200	NDT	54 ± 9%
RIP NP, H-2^d IFN-γ–competent	Spleen	1/3,000	1/9,000	1/3,900	NDT	34 ± 8%
RIP NP, H-2^d IFN-γ–deficient	Spleen	1/5,000	1/9,000	1/7,000	NDT	35 ± 2%
RIP NP, IDDM IFN-γ–competent	Pancreas	1/2,000	not done	1/6,000	NDT	38 ± 7%
RIP NP, no IDDM γ-IFN–deficient	Pancreas	1/20,000	NDT	NDT	NDT	18 ± 5%
Secondary CTLs were recovered from spleens and pancreata of infected (10³ PFU LCMV intraperitoneally) IFN-γ–deficient RIP LCMV mice, single transgenic RIP LCMV mice, and nontransgenic IFN-γ–deficient or –competent controls. CTL activity was tested on syngeneic LCMV infected or uninfected target cells after a 5–14 d in vitro stimulation (see Materials and Methods), and precursor frequency analysis was performed as described (26). NDT, not detectable (<1/60,000).

		Precursor frequency of CTL				Killing of H-2^d targets
Secondary effector day 60 CTL		H-2^d			H-2^b	Killing of H-2^d targets
Group	Origin	LCMV	vv/GP	NPpep	LCMV	LCMV
H-2^d IFN-γ–competent	Spleen	1/1,500	NDT	1/2,500	NDT	60 ± 7%
H-2^d IFN-γ–deficient	Spleen	1/3,000	NDT	1/4,200	NDT	54 ± 9%
RIP NP, H-2^d IFN-γ–competent	Spleen	1/3,000	1/9,000	1/3,900	NDT	34 ± 8%
RIP NP, H-2^d IFN-γ–deficient	Spleen	1/5,000	1/9,000	1/7,000	NDT	35 ± 2%
RIP NP, IDDM IFN-γ–competent	Pancreas	1/2,000	not done	1/6,000	NDT	38 ± 7%
RIP NP, no IDDM γ-IFN–deficient	Pancreas	1/20,000	NDT	NDT	NDT	18 ± 5%
Secondary CTLs were recovered from spleens and pancreata of infected (10³ PFU LCMV intraperitoneally) IFN-γ–deficient RIP LCMV mice, single transgenic RIP LCMV mice, and nontransgenic IFN-γ–deficient or –competent controls. CTL activity was tested on syngeneic LCMV infected or uninfected target cells after a 5–14 d in vitro stimulation (see Materials and Methods), and precursor frequency analysis was performed as described (26). NDT, not detectable (<1/60,000).