Skip to Main Content
Table 1

Levels of IL-2 Production by Spleen Cells from SCID Mice Transplanted with Fetal Liver Cells in Response to Various Stimuli

relA genotype of fetal liver donor*Stimuli
Con AAnti-CD3Anti-CD28Anti-CD3 + Anti-CD28PMA + calcium ionophoreNone
      IL-2 levels     
      U/ml§     
+/+  167 ± 16.2  63.7 ± 10.3  5.7 ± 0.84  446 ± 19.8  361 ± 69.8  4.5 ± 1.42 
+/−  146 ± 18.5  42.8 ± 5.27  5.2 ± 0.82  448 ± 10.9  312 ± 26.1  2.0 ± 0.05 
−/−  118 ± 16.8  60.3 ± 8.11  6.5 ± 1.08  402 ± 88.0  276 ± 42.3  2.4 ± 0.32 
None     1.5 ± 0.66   3.4 ± 1.03  2.3 ± 0.62     5.2 ± 1.84     6.5 ± 1.32  1.4 ± 0.32 
relA genotype of fetal liver donor*Stimuli
Con AAnti-CD3Anti-CD28Anti-CD3 + Anti-CD28PMA + calcium ionophoreNone
      IL-2 levels     
      U/ml§     
+/+  167 ± 16.2  63.7 ± 10.3  5.7 ± 0.84  446 ± 19.8  361 ± 69.8  4.5 ± 1.42 
+/−  146 ± 18.5  42.8 ± 5.27  5.2 ± 0.82  448 ± 10.9  312 ± 26.1  2.0 ± 0.05 
−/−  118 ± 16.8  60.3 ± 8.11  6.5 ± 1.08  402 ± 88.0  276 ± 42.3  2.4 ± 0.32 
None     1.5 ± 0.66   3.4 ± 1.03  2.3 ± 0.62     5.2 ± 1.84     6.5 ± 1.32  1.4 ± 0.32 
*

 3 × 106 fetal liver cells from relA +/+, +/−, or −/− embryos were transplanted into irradiated SCID mice.  

 3 × 105 spleen cells were stimulated as described in the Materials and Methods.  

§

 The levels of IL-2 were measured by bioassay using NRB cells (see the Materials and Methods).  

 Mean ± SD. The results obtained from three mice were averaged.  

Close Modal

or Create an Account

Close Modal
Close Modal