Rockefeller University Press

Table 2

Grouping of Patient Isolate env Clones within pCR3 Based upon Restriction Digests and Choice of Predominant Clones for Preparation of vv

Patient		Age		Group		Env clones^*		Recombinant vv
VI-05		2 mo		05-2M-A		3-2, 3-4, 3-5, 3-7, 3-8, 3-10		vvVI-05-2M
				05-2M-B		3-3
		6 mo		05-6M-A		4-2, 4-4, 4-5, 4-6, 4-8, 4-9		vvVI-05-6M
VI-06		Birth (1 d)		06-1D-A		1-2, 1-3
				06-1D-B		1-9
				06-1D-C		1-11, 1-12, 1-13, 1-15, 1-20		vvVI-06-ID
				06-1D-D		1-18
		6 mo		06-6M-A		2-1, 2-2, 2-3, 2-4, 2-7, 2-8, 2-9, 2-10		^‡
				06-6M-B		2-5		^‡
VI-08		20 d		08-20D-A		5-4
				08-20D-B		5-6
				08-20D-C		5-2, 5-3, 5-8, 5-9, 5-10		vvVI-08-20D
		6 mo		08-6M-A		6-1
				08-6M-B		6-2, 6-3, 6-4, 6-7, 6-8, 6-9, 6-10		vvVI-08-6M
VI-11		1 mo		11-1M-A		7-1
				11-1M-B		7-2, 7-5, 7-7, 7-10, 7-11, 7-16, 7-19		vvVI-11-1M
				11-1M-C		7-12
		3 mo		11-3M-A		8-3
				11-3M-B		8-4, 8-11, 8-14, 8-16		vvVI-11-3M

Patient		Age		Group		Env clones^*		Recombinant vv
VI-05		2 mo		05-2M-A		3-2, 3-4, 3-5, 3-7, 3-8, 3-10		vvVI-05-2M
				05-2M-B		3-3
		6 mo		05-6M-A		4-2, 4-4, 4-5, 4-6, 4-8, 4-9		vvVI-05-6M
VI-06		Birth (1 d)		06-1D-A		1-2, 1-3
				06-1D-B		1-9
				06-1D-C		1-11, 1-12, 1-13, 1-15, 1-20		vvVI-06-ID
				06-1D-D		1-18
		6 mo		06-6M-A		2-1, 2-2, 2-3, 2-4, 2-7, 2-8, 2-9, 2-10		^‡
				06-6M-B		2-5		^‡
VI-08		20 d		08-20D-A		5-4
				08-20D-B		5-6
				08-20D-C		5-2, 5-3, 5-8, 5-9, 5-10		vvVI-08-20D
		6 mo		08-6M-A		6-1
				08-6M-B		6-2, 6-3, 6-4, 6-7, 6-8, 6-9, 6-10		vvVI-08-6M
VI-11		1 mo		11-1M-A		7-1
				11-1M-B		7-2, 7-5, 7-7, 7-10, 7-11, 7-16, 7-19		vvVI-11-1M
				11-1M-C		7-12
		3 mo		11-3M-A		8-3
				11-3M-B		8-4, 8-11, 8-14, 8-16		vvVI-11-3M

env genes amplified by PCR and ligated into pCR3 (InVitrogen); clones chosen for preparation of vv are in bold type.

vv not made from this time point due to instability of the env insert.