Immunization of Balb/c Mice with Affinity-purified Recombinant NspA Protein Confers Protection against Subsequent Challenge with N. meningitidis Strain 608B
| . | . | Number of living mice after the bacterial challenge* . | . | . | ||
|---|---|---|---|---|---|---|
| Groups . | . | 24 h . | . | 72 h . | . | % Survival . |
| 10 μg of purified NspA protein | 20/20 | 16/20 | 80 | |||
| 20 μg of purified NspA protein | 9/10 | 8/10 | 80 | |||
| 20 μg of BSA | 6/10 | 2/10 | 20 | |||
| 20 μg of concentrated E. coli supernatant | 7/10 | 2/10 | 20 | |||
| Phosphate buffered saline | 8/10 | 0/10 | 0 | |||
| . | . | Number of living mice after the bacterial challenge* . | . | . | ||
|---|---|---|---|---|---|---|
| Groups . | . | 24 h . | . | 72 h . | . | % Survival . |
| 10 μg of purified NspA protein | 20/20 | 16/20 | 80 | |||
| 20 μg of purified NspA protein | 9/10 | 8/10 | 80 | |||
| 20 μg of BSA | 6/10 | 2/10 | 20 | |||
| 20 μg of concentrated E. coli supernatant | 7/10 | 2/10 | 20 | |||
| Phosphate buffered saline | 8/10 | 0/10 | 0 | |||
The mice were challenged with 1 ml of a suspension containing 1,000 CFU of N. meningitidis strain 608B, 4% mucin (Sigma), and 1.6% hemoglobin (Oxoid).