Effect of MPIF-1 and MPIF-2 on the Murine Hematopoietic Progenitor Cell Colony Formation In Vitro in Response to Known Cytokines
| Additions . | . | CFU-E . | . | Progenitor colonies . | . | LPP-CFC . | ||||
|---|---|---|---|---|---|---|---|---|---|---|
| BFU-E . | . | CFU-GM . | . | HPP-CFC . | ||||||
| CC | 94 ± 10 | 13 ± 2.5 | 29 ± 3.0 | 35 ± 4.0 | 114 ± 12 | |||||
| +MPIF-1 | 89 ± 11 | 12 ± 2.0 | 11 ± 2.0 | 29 ± 2.0 | 27 ± 6.0 | |||||
| +MPIF-2 | 91 ± 4.0 | 12 ± 3.0 | 29 ± 2.0 | 8 ± 2.0 | 109 ± 14 | |||||
| +MCP-4 | 91 ± 11 | 12 ± 3.0 | 31 ± 4.0 | 34 ± 2.0 | 116 ± 17 | |||||
| Additions . | . | CFU-E . | . | Progenitor colonies . | . | LPP-CFC . | ||||
|---|---|---|---|---|---|---|---|---|---|---|
| BFU-E . | . | CFU-GM . | . | HPP-CFC . | ||||||
| CC | 94 ± 10 | 13 ± 2.5 | 29 ± 3.0 | 35 ± 4.0 | 114 ± 12 | |||||
| +MPIF-1 | 89 ± 11 | 12 ± 2.0 | 11 ± 2.0 | 29 ± 2.0 | 27 ± 6.0 | |||||
| +MPIF-2 | 91 ± 4.0 | 12 ± 3.0 | 29 ± 2.0 | 8 ± 2.0 | 109 ± 14 | |||||
| +MCP-4 | 91 ± 11 | 12 ± 3.0 | 31 ± 4.0 | 34 ± 2.0 | 116 ± 17 | |||||
CC, appropriate cocktail of cytokines were used as described in Materials and Methods. Chemokines were added at 50 ng/ml final concentration. Data shown are pooled from two separate experiments and are expressed as mean ± SD.