Effect of MPIF-1 and MPIF-2 on Human Hematopoietic Progenitor Colony Formation In Vitro
| Experiment . | . | Additions . | . | BFU-E . | . | CFU-G . | . | CFU-M . | . | CFU-GM . | . | CFU-Meg . | . | CFU-Mix . |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Colonies/103 cells | ||||||||||||||
| 1 | CC | 13 ± 2 | 15 ± 2 | 14 ± 3 | 16 ± 3 | 11 ± 3 | 11 ± 2 | |||||||
| + MPIF-1 | 19 ± 2 | 18 ± 5 | 12 ± 2 | 8 ± 2 | 12 ± 2 | 5 ± 1 | ||||||||
| + MPIF-2 | 16 ± 4 | 12 ± 2 | 13 ± 1 | 9 ± 3 | 14 ± 3 | 3 ± 1 | ||||||||
| + MIP-1α | 17 ± 3 | 19 ± 5 | 14 ± 2 | 14 ± 4 | 12 ± 3 | 12 ± 2 | ||||||||
| 2 | CC | 14 ± 3 | 13 ± 3 | 13 ± 1 | 12 ± 3 | 13 ± 1 | 11 ± 2 | |||||||
| + MPIF-1 | 14 ± 2 | 11 ± 1 | 12 ± 2 | 5 ± 1 | 12 ± 2 | 4 ± 1 | ||||||||
| + MPIF-2 | 13 ± 2 | 12 ± 1 | 13 ± 1 | 8 ± 2 | 12 ± 3 | 6 ± 2 | ||||||||
| + MIP-1α | 12 ± 2 | 12 ± 3 | 13 ± 2 | 13 ± 2 | 14 ± 2 | 12 ± 1 | ||||||||
| + MCP-4 | 12 ± 1 | 14 ± 2 | 12 ± 1 | 12 ± 2 | 11 ± 1 | 12 ± 2 | ||||||||
| Experiment . | . | Additions . | . | BFU-E . | . | CFU-G . | . | CFU-M . | . | CFU-GM . | . | CFU-Meg . | . | CFU-Mix . |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Colonies/103 cells | ||||||||||||||
| 1 | CC | 13 ± 2 | 15 ± 2 | 14 ± 3 | 16 ± 3 | 11 ± 3 | 11 ± 2 | |||||||
| + MPIF-1 | 19 ± 2 | 18 ± 5 | 12 ± 2 | 8 ± 2 | 12 ± 2 | 5 ± 1 | ||||||||
| + MPIF-2 | 16 ± 4 | 12 ± 2 | 13 ± 1 | 9 ± 3 | 14 ± 3 | 3 ± 1 | ||||||||
| + MIP-1α | 17 ± 3 | 19 ± 5 | 14 ± 2 | 14 ± 4 | 12 ± 3 | 12 ± 2 | ||||||||
| 2 | CC | 14 ± 3 | 13 ± 3 | 13 ± 1 | 12 ± 3 | 13 ± 1 | 11 ± 2 | |||||||
| + MPIF-1 | 14 ± 2 | 11 ± 1 | 12 ± 2 | 5 ± 1 | 12 ± 2 | 4 ± 1 | ||||||||
| + MPIF-2 | 13 ± 2 | 12 ± 1 | 13 ± 1 | 8 ± 2 | 12 ± 3 | 6 ± 2 | ||||||||
| + MIP-1α | 12 ± 2 | 12 ± 3 | 13 ± 2 | 13 ± 2 | 14 ± 2 | 12 ± 1 | ||||||||
| + MCP-4 | 12 ± 1 | 14 ± 2 | 12 ± 1 | 12 ± 2 | 11 ± 1 | 12 ± 2 | ||||||||
CC, a cocktail of cytokines was added as described in Materials and Methods. Chemokines were added at 50 ng/ml final concentration. Data from two representative experiments performed in triplicates are shown and are expressed as mean ± SD.