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Table 2

Mating Behavior and Reproductive System in Male Mice

Genotype+/++/−−/−
Mating behavior       
Mounting       
 Latency (min)  5.3 ± 2.3 (5)  16.3 ± 7.1 (5)  *29.5 ± 10.5 (5) 
 Appropriate (count)  25.2 ± 6.7 (5)  18.3 ± 1.4 (5)  *4.4 ± 3.9 (5) 
 Inappropriate (count)   3.0 ± 1.4 (5)   2.8 ± 0.4 (5)        4.6 ± 3.0 (5) 
Total contact time (min)  52.2 ± 15.1 (5)  52.7 ± 9.5 (5)       41.7 ± 13.5 (5) 
Intromission (count)  12.3 ± 8.8 (5)   3.3 ± 0.5 (5)  *0.8 ± 0.8 (5) 
Ejaculating during the test  40% (5)  *0% (5)  *0% (5) 
Overnight plug  60% (5)  40% (5)  *20% (5) 
Parameters of male reproductive system     
Sperm count (× 106 epididymis)  10.9 ± 1.4 (4)  12.1 ± 0.6 (4)  *7.0 ± 2.3 (4) 
Sperm motility (%)  78.4 ± 2.2 (4)  74.0 ± 1.3 (4)  *54.5 ± 7.4 (4) 
Ventral prostate weight (mg/g weight)  0.46 ± 0.13 (4)  0.47 ± 0.10 (4)       0.47 ± 0.05 (4) 
Seminal vesicle weight (mg/g body weight)  6.8 ± 0.6 (4)   9.3 ± 1.5 (4)        8.2 ± 0.8 (4) 
Time to pregnancy (d)  1.3 ± 0.2 (6)   3.2 ± 0.8 (15)        *5.1 ± 0.5 (22) 
Offspring litter size  9.3 ± 0.6 (8)        8.1 ± 0.5 (23)        *6.0 ± 0.3 (34) 
Genotype+/++/−−/−
Mating behavior       
Mounting       
 Latency (min)  5.3 ± 2.3 (5)  16.3 ± 7.1 (5)  *29.5 ± 10.5 (5) 
 Appropriate (count)  25.2 ± 6.7 (5)  18.3 ± 1.4 (5)  *4.4 ± 3.9 (5) 
 Inappropriate (count)   3.0 ± 1.4 (5)   2.8 ± 0.4 (5)        4.6 ± 3.0 (5) 
Total contact time (min)  52.2 ± 15.1 (5)  52.7 ± 9.5 (5)       41.7 ± 13.5 (5) 
Intromission (count)  12.3 ± 8.8 (5)   3.3 ± 0.5 (5)  *0.8 ± 0.8 (5) 
Ejaculating during the test  40% (5)  *0% (5)  *0% (5) 
Overnight plug  60% (5)  40% (5)  *20% (5) 
Parameters of male reproductive system     
Sperm count (× 106 epididymis)  10.9 ± 1.4 (4)  12.1 ± 0.6 (4)  *7.0 ± 2.3 (4) 
Sperm motility (%)  78.4 ± 2.2 (4)  74.0 ± 1.3 (4)  *54.5 ± 7.4 (4) 
Ventral prostate weight (mg/g weight)  0.46 ± 0.13 (4)  0.47 ± 0.10 (4)       0.47 ± 0.05 (4) 
Seminal vesicle weight (mg/g body weight)  6.8 ± 0.6 (4)   9.3 ± 1.5 (4)        8.2 ± 0.8 (4) 
Time to pregnancy (d)  1.3 ± 0.2 (6)   3.2 ± 0.8 (15)        *5.1 ± 0.5 (22) 
Offspring litter size  9.3 ± 0.6 (8)        8.1 ± 0.5 (23)        *6.0 ± 0.3 (34) 

Mating was measured over a 2-h period. Mounting latency was the time from when an estrous female mouse was introduced into the cage to when the male first attempted mounting. Appropriate mounting, mounting attempt oriented toward the rear of the female; inappropriate mounting, attempt oriented elsewhere (side, front, etc.); total contact time, accumulative time when the male was investigating the female mouse, including mounting and sniffing of body and genital areas. Ejaculation was determined at the end of the test by inspection of the female's vagina for the presence of a seminal plug. Overnight plug was determined by overnight housing of individual male mice after the testing with an estrous female mouse and scoring the presence of a seminal plug. Reproductive system parameters were measured as described in Materials and Methods. The numbers in parentheses represent data shown as mean ± SEM.  

*

 Significant difference (P <0.05, Student's t test) from the wild-type value.  

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