Table 3

CLL Samples That Lack Ig Heavy Chain Allelic Exclusion

SampleVH genePercent homologyAnticipated phenotypeSpAB6Lc1μ
P1  VH3-33   99  SA+  N.T.       
  VH4-39   98  Lc1+      N.T.   
P2  VH3-7   97  SA+/B6+  2.4  1.4     
  VH4-39   99  Lc1+      10  9.5 
P3  VH2-70   88  –         
  VH3-7  100  SA+/B6+  1.9  1.4     
  VH4-59   99  Lc1+      1.6  1.9 
P4  VH3-30   99  SA+/B6+   1.8     
  VH4-b   90  Lc1+      9.4  7.2 
P5  VH3-23   98  SA+/B6+  1.8  2.1     
  VH4-61   96  Lc1+      4.9  7.4 
P6  VH2-70   90  –         
  VH3-30.3   88  SA+/B6+    N.T.  N.T.   
SampleVH genePercent homologyAnticipated phenotypeSpAB6Lc1μ
P1  VH3-33   99  SA+  N.T.       
  VH4-39   98  Lc1+      N.T.   
P2  VH3-7   97  SA+/B6+  2.4  1.4     
  VH4-39   99  Lc1+      10  9.5 
P3  VH2-70   88  –         
  VH3-7  100  SA+/B6+  1.9  1.4     
  VH4-59   99  Lc1+      1.6  1.9 
P4  VH3-30   99  SA+/B6+   1.8     
  VH4-b   90  Lc1+      9.4  7.2 
P5  VH3-23   98  SA+/B6+  1.8  2.1     
  VH4-61   96  Lc1+      4.9  7.4 
P6  VH2-70   90  –         
  VH3-30.3   88  SA+/B6+    N.T.  N.T.   

The far left column (Sample) provides the designator for each sample (P1–P6). The second column (VH gene) lists the germline Ig VH genes that have the highest homology with the Ig VH genes isolated from each sample. The percent homology column lists the percent sequence homology between the isolated VH gene and the deduced germline VH gene having the highest base sequence homology. The Anticipated Phenotype column provides the expected reactivity of the surface Ig with the anti-CRI or anti-VH subgroup reagents. The columns labeled SpA, B6, Lc1, or μ provide the observed MFIR of the leukemia cell population stained with each of these reagents relative to that of the cells stained with an isotype control mAb. N.T., sample was not tested.  

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