IL-12 and IFN-γ Synthesis by Cells Harvested from iNOS ko and C57BL/6 Mice at 5 d after i.p. Challenge with T. gondii
| . | . | . | . | . | . | Peritoneal exudate . | . | Spleen . | . | Serum . | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | . | . | . | . | . | Mean ± SE . | . | n . | . | Range . | . | Mean ± SE . | . | n . | . | Range . | . | Mean ± SE . | . | n . | . | Range . |
| IL-12p40 | C57BL/6 | bkg | 1.4 ± 0.5 | 6 | 0–3.5 | 5.4 ± 1.8 | 7 | 2.3–12.7 | 17.1 ± 3.9 | 3 | 15.6–23.2 | |||||||||||
| STAg | 10.9 ± 3.3 | 6 | 0.3–19.5 | 35.4 ± 6.0 | 7 | 20.7–53.5 | ||||||||||||||||
| iNOS ko | bkg | 1.9 ± 0.4 | 6 | 1.2–4.1 | 10.3 ± 2.8 | 9 | 3.6–17.6 | 19.5 ± 9 | 3 | 13.7–27.3 | ||||||||||||
| STAg | 11.9 ± 4.3 | 6 | 4.3 ± 25.9 | 56.4 ± 9.1 | 9 | 38.0–93.6 | ||||||||||||||||
| IFN-γ | C57BL/6 | bkg | 0.1 ± 0.06 | 6 | 0–0.3 | 0.6 ± 0.5 | 7 | 0–3.1 | 5.1 ± 0.5 | 3 | 4.4–5.8 | |||||||||||
| STAg | 1.0 ± 0.3 | 6 | 0.2–2.1 | 8.3 ± 2.1 | 7 | 1.3–17.9 | ||||||||||||||||
| iNOS ko | bkg | 6.0 ± 2.9 | 6 | 0.3–15.4 | 1.1 ± 0.7 | 9 | 0–3.9 | 3.4 ± 0.3 | 3 | 2.9–3.8 | ||||||||||||
| STAg | 8.2 ± 4.0 | 6 | 0.8–2.13 | 10.4 ± 2.7 | 9 | 1.8–19.2 | ||||||||||||||||
| . | . | . | . | . | . | Peritoneal exudate . | . | Spleen . | . | Serum . | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | . | . | . | . | . | Mean ± SE . | . | n . | . | Range . | . | Mean ± SE . | . | n . | . | Range . | . | Mean ± SE . | . | n . | . | Range . |
| IL-12p40 | C57BL/6 | bkg | 1.4 ± 0.5 | 6 | 0–3.5 | 5.4 ± 1.8 | 7 | 2.3–12.7 | 17.1 ± 3.9 | 3 | 15.6–23.2 | |||||||||||
| STAg | 10.9 ± 3.3 | 6 | 0.3–19.5 | 35.4 ± 6.0 | 7 | 20.7–53.5 | ||||||||||||||||
| iNOS ko | bkg | 1.9 ± 0.4 | 6 | 1.2–4.1 | 10.3 ± 2.8 | 9 | 3.6–17.6 | 19.5 ± 9 | 3 | 13.7–27.3 | ||||||||||||
| STAg | 11.9 ± 4.3 | 6 | 4.3 ± 25.9 | 56.4 ± 9.1 | 9 | 38.0–93.6 | ||||||||||||||||
| IFN-γ | C57BL/6 | bkg | 0.1 ± 0.06 | 6 | 0–0.3 | 0.6 ± 0.5 | 7 | 0–3.1 | 5.1 ± 0.5 | 3 | 4.4–5.8 | |||||||||||
| STAg | 1.0 ± 0.3 | 6 | 0.2–2.1 | 8.3 ± 2.1 | 7 | 1.3–17.9 | ||||||||||||||||
| iNOS ko | bkg | 6.0 ± 2.9 | 6 | 0.3–15.4 | 1.1 ± 0.7 | 9 | 0–3.9 | 3.4 ± 0.3 | 3 | 2.9–3.8 | ||||||||||||
| STAg | 8.2 ± 4.0 | 6 | 0.8–2.13 | 10.4 ± 2.7 | 9 | 1.8–19.2 | ||||||||||||||||
Mice were infected with 20 ME49 cysts each, killed 5 d later, and then peritoneal and spleen cells were isolated. Cell suspensions from each animal were cultured for 72 h in medium alone or in the presence of STAG (5 μg/ml). Supernatants were then assayed by ELISA for IL-12p40 and IFN-γ as described in Materials and Methods. The data shown are pooled from three experiments employing the number of animals indicated.