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Table 2

In Vitro Control of T. gondii Replication by Peritoneal Exudate Cells Harvested from Naive and 5-d Infected iNOS ko or C57BL/6 Mice

In vitro stimulusTachyzoite/cell ratioNormal resident cellsT. gondii elicited cells
C57BL/6iNOS koC57BL/6iNOS ko
cpm% killingcpm% killingcpm% killingcpm% killing
None    43 ± 22    74 ± 53    294 ± 49    181 ± 21   
None  0.2  3146 ± 478     2746 ± 660    225 ± 74    1866 ± 44   
None   7554 ± 202    8654 ± 1489    226 ± 39    4882 ± 815   
                   
IFN-γ    58 ± 15    54 ± 20    125 ± 26    97 ± 9   
IFN-γ  0.2  300 ± 33  92.2   1827 ± 317  31.0  205 ± 90   3031 ± 88  
IFN-γ   1180 ± 126  85.1   5997 ± 892  29.9  188 ± 35   5023 ± 822  
In vitro stimulusTachyzoite/cell ratioNormal resident cellsT. gondii elicited cells
C57BL/6iNOS koC57BL/6iNOS ko
cpm% killingcpm% killingcpm% killingcpm% killing
None    43 ± 22    74 ± 53    294 ± 49    181 ± 21   
None  0.2  3146 ± 478     2746 ± 660    225 ± 74    1866 ± 44   
None   7554 ± 202    8654 ± 1489    226 ± 39    4882 ± 815   
                   
IFN-γ    58 ± 15    54 ± 20    125 ± 26    97 ± 9   
IFN-γ  0.2  300 ± 33  92.2   1827 ± 317  31.0  205 ± 90   3031 ± 88  
IFN-γ   1180 ± 126  85.1   5997 ± 892  29.9  188 ± 35   5023 ± 822  

Mice (3–5 per group) were infected with 20 ME49 cysts each and 5 d later the animals were killed and peritoneal cells were isolated and pooled. The cells were next precultured in the presence or absence of IFN-γ, infected and harvested as described in Table 1. Percentage killing of tachyzoites was then calculated. The experiment shown is representative of four performed.  

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