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Table 1

Summary of Adoptive Transfer Experiments

Donor anti-HEL IgTg B cellsRecipientResults
Location of transferred B cells in spleen (day 1)Corresponding figure
MHCBCR ligation*StrainFollicular repertoireTg T cell help
H-2b   Non-Tg  Diverse  −  Follicle       1 A 
  1 ng/ml  Non-Tg  Diverse  −  Follicle       1 B 
  20 ng/ml  Non-Tg  Diverse  −  Follicle/outer PALS       1 C 
  100 ng/ml  Non-Tg  Diverse  −  Outer PALS       1 D 
H-2bk  100 ng/ml  Non-Tg  Diverse   Outer PALS       2, A–C 
   HEL Tg  Diverse   Outer PALS       2, D–F 
   Non-Tg  Diverse   Follicle       2, G–I 
H-2b   IgTg  Monoclonal  −  Follicle         3 A 
  100 ng/ml  IgTg  Monoclonal  −  Outer PALS         3 B 
   Double Tg  Monoclonal  −  Follicle         3 C 
  100 ng/ml  Double Tg  Monoclonal  −  Outer PALS         3 D 
Donor anti-HEL IgTg B cellsRecipientResults
Location of transferred B cells in spleen (day 1)Corresponding figure
MHCBCR ligation*StrainFollicular repertoireTg T cell help
H-2b   Non-Tg  Diverse  −  Follicle       1 A 
  1 ng/ml  Non-Tg  Diverse  −  Follicle       1 B 
  20 ng/ml  Non-Tg  Diverse  −  Follicle/outer PALS       1 C 
  100 ng/ml  Non-Tg  Diverse  −  Outer PALS       1 D 
H-2bk  100 ng/ml  Non-Tg  Diverse   Outer PALS       2, A–C 
   HEL Tg  Diverse   Outer PALS       2, D–F 
   Non-Tg  Diverse   Follicle       2, G–I 
H-2b   IgTg  Monoclonal  −  Follicle         3 A 
  100 ng/ml  IgTg  Monoclonal  −  Outer PALS         3 B 
   Double Tg  Monoclonal  −  Follicle         3 C 
  100 ng/ml  Double Tg  Monoclonal  −  Outer PALS         3 D 
*

 In vitro pulse with HEL.   

 In vitro pulse with MCC87-103 to attract T cell help.  

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