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Table 2

Summary of Experiments Investigating B cell Location in Double Transgenic Mice

Anti-HEL/HEL double Tg lineDeterminant of effective serum HEL concentrationB cell repertoireLocation of IgTg B cells in spleenCorresponding figure
IgTg × ML5  Zinc induction of HEL  Predominantly tolerant IgTg (monoclonal)     
   Day 1 and 2: baseline   Follicle  4 A 
   Day 3 and 4: induced    Outer PALS  4 B 
IgTg × AL3  Sex  Predominantly tolerant  IgTg (monoclonal)     
   M ∼80 ng/ml   Follicle  4 C 
   F ∼160 ng/ml    Outer PALS  4 D 
BM chimaeras IgTg/non-Tg BM into ML5  Proportion of HEL-binding  B cells in repertoire  % HEL-binding B cells     
100:0    94  Follicle   
99:1    63  Follicle   
95:5    13  Follicle/outer PALS  4 E 
80:20     Outer PALS  4 F 
Anti-HEL/HEL double Tg lineDeterminant of effective serum HEL concentrationB cell repertoireLocation of IgTg B cells in spleenCorresponding figure
IgTg × ML5  Zinc induction of HEL  Predominantly tolerant IgTg (monoclonal)     
   Day 1 and 2: baseline   Follicle  4 A 
   Day 3 and 4: induced    Outer PALS  4 B 
IgTg × AL3  Sex  Predominantly tolerant  IgTg (monoclonal)     
   M ∼80 ng/ml   Follicle  4 C 
   F ∼160 ng/ml    Outer PALS  4 D 
BM chimaeras IgTg/non-Tg BM into ML5  Proportion of HEL-binding  B cells in repertoire  % HEL-binding B cells     
100:0    94  Follicle   
99:1    63  Follicle   
95:5    13  Follicle/outer PALS  4 E 
80:20     Outer PALS  4 F 
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