Rockefeller University Press

Table 3

Association (K_on) and Dissociation (K_off) Rates of sIL-4R Allotypes Evaluated with Surface Plasmon Resonance

sIL-4R		K_on (M⁻¹ s⁻¹)	K_off (s⁻¹)	K_d (nM)
C57BL/6	Exp. 1	5.06 × 10⁴	1.48 × 10⁻³	29.25
	Exp. 2	4.33 × 10⁴	1.30 × 10⁻³	30.02
BALB/c	Exp. 1	8.73 × 10⁴	2.07 × 10⁻³	23.71
	Exp. 2	8.54 × 10⁴	2.08 × 10⁻³	24.36

sIL-4R		K_on (M⁻¹ s⁻¹)	K_off (s⁻¹)	K_d (nM)
C57BL/6	Exp. 1	5.06 × 10⁴	1.48 × 10⁻³	29.25
	Exp. 2	4.33 × 10⁴	1.30 × 10⁻³	30.02
BALB/c	Exp. 1	8.73 × 10⁴	2.07 × 10⁻³	23.71
	Exp. 2	8.54 × 10⁴	2.08 × 10⁻³	24.36

Binding of sIL-4R allotypes to immobilized IL-4 and resulting K_on and K_off rates obtained with two independent protein preparations (Exp. 1 and 2). The affinity constant (K_d) is calculated by the equation K_off/K_on.

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