Rockefeller University Press

Table 1

Phenotypic Characterization of CD8⁺ T Cell Subsets^*

	CD45RA⁺CD27⁺	CD45RA⁺CD27⁻	CD45RA⁻CD27⁺	CD45RA⁻CD27⁻
Homing receptors
CD62L	76 ± 9^‡	28 ± 13^‖	20 ± 7^‖	30 ± 3^‖
Integrins
CD49d	25 ± 13	87 ± 2^‖	41 ± 15^‖	34 ± 20
CD49e	25 ± 14	76 ± 12^‖	56 ± 6^‖	61 ± 12^‖
CD29	27 ± 15	93 ± 2^‖	73 ± 6^‖	83 ± 3^‖
CD11a	290 ± 60^§	716 ± 50^‖	369 ± 100	317 ± 100
CD18^bright	21 ± 20	87 ± 7^‖	47 ± 17	32 ± 18
CD11b	10 ± 7	67 ± 13^‖	9 ± 3	25 ± 3^‖

Activation and costimulatory molecules
CD58	18 ± 16	86 ± 8^‖	80 ± 8^‖	88 ± 10^‖
CD28	77 ± 12	17 ± 4^‖	83 ± 2	50 ± 7^‖
CD57	1.0 ± 0.7	50 ± 13^‖	10 ± 6^‖	14 ± 9^‖
CD95	10 ± 1^§	29 ± 8^‖	56 ± 2^‖	58 ± 10^‖

	CD45RA⁺CD27⁺	CD45RA⁺CD27⁻	CD45RA⁻CD27⁺	CD45RA⁻CD27⁻
Homing receptors
CD62L	76 ± 9^‡	28 ± 13^‖	20 ± 7^‖	30 ± 3^‖
Integrins
CD49d	25 ± 13	87 ± 2^‖	41 ± 15^‖	34 ± 20
CD49e	25 ± 14	76 ± 12^‖	56 ± 6^‖	61 ± 12^‖
CD29	27 ± 15	93 ± 2^‖	73 ± 6^‖	83 ± 3^‖
CD11a	290 ± 60^§	716 ± 50^‖	369 ± 100	317 ± 100
CD18^bright	21 ± 20	87 ± 7^‖	47 ± 17	32 ± 18
CD11b	10 ± 7	67 ± 13^‖	9 ± 3	25 ± 3^‖

Activation and costimulatory molecules
CD58	18 ± 16	86 ± 8^‖	80 ± 8^‖	88 ± 10^‖
CD28	77 ± 12	17 ± 4^‖	83 ± 2	50 ± 7^‖
CD57	1.0 ± 0.7	50 ± 13^‖	10 ± 6^‖	14 ± 9^‖
CD95	10 ± 1^§	29 ± 8^‖	56 ± 2^‖	58 ± 10^‖

CD8⁺ cells were >97% CD3⁺TCR-α/β⁺CD16⁻ in all subsets.

Percentage of positive cells ± SD, n = 5.

Mean fluorescence intensity ± SD, n = 5.

Significant difference (P <0.05) compared to the CD45RA⁺CD27⁺ subset (Wilcoxon signed rank test).