Rockefeller University Press

Table 2

Intracellular Measurement of Cytokine Production in CD8⁺ T Cell Subsets

Cells	IL-2^*	IL-2^‡	IFN-γ≳	TNF-α≳	IL-4^*
Total CD8⁺	17 ± 2	16	51 ± 15	48 ± 14	3 ± 1
CD45RA⁺CD27⁺	9 ± 4	27	6 ± 2	12 ± 4	<1
CD45RA⁺CD27⁻	1 ± 1	<1	77 ± 16	69 ± 10	<1
CD45RA⁻CD27⁺	43 ± 17	31	76 ± 9	78 ± 8	6 ± 4
CD45RA⁻CD27⁻	33 ± 14	16	76 ± 12	69 ± 6	9 ± 8

Cells	IL-2^*	IL-2^‡	IFN-γ≳	TNF-α≳	IL-4^*
Total CD8⁺	17 ± 2	16	51 ± 15	48 ± 14	3 ± 1
CD45RA⁺CD27⁺	9 ± 4	27	6 ± 2	12 ± 4	<1
CD45RA⁺CD27⁻	1 ± 1	<1	77 ± 16	69 ± 10	<1
CD45RA⁻CD27⁺	43 ± 17	31	76 ± 9	78 ± 8	6 ± 4
CD45RA⁻CD27⁻	33 ± 14	16	76 ± 12	69 ± 6	9 ± 8

Cells were stimulated for 4 h, mean percentage of positive cells ± SD, n = 6.

Cells were stimulated for 8 h, mean percentage of positive cells, n = 2.