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Table 2

Intracellular Measurement of Cytokine Production in CD8+ T Cell Subsets

CellsIL-2*IL-2IFN-γ≳TNF-α≳IL-4*
Total CD8+  17 ± 2    16  51 ± 15   48 ± 14  3 ± 1 
CD45RA+CD27+  9 ± 4    27  6 ± 2   12 ± 4  <1 
CD45RA+CD27  1 ± 1  <1  77 ± 16   69 ± 10  <1 
CD45RACD27+  43 ± 17    31  76 ± 9   78 ± 8  6 ± 4 
CD45RACD27  33 ± 14    16  76 ± 12   69 ± 6  9 ± 8 
CellsIL-2*IL-2IFN-γ≳TNF-α≳IL-4*
Total CD8+  17 ± 2    16  51 ± 15   48 ± 14  3 ± 1 
CD45RA+CD27+  9 ± 4    27  6 ± 2   12 ± 4  <1 
CD45RA+CD27  1 ± 1  <1  77 ± 16   69 ± 10  <1 
CD45RACD27+  43 ± 17    31  76 ± 9   78 ± 8  6 ± 4 
CD45RACD27  33 ± 14    16  76 ± 12   69 ± 6  9 ± 8 
*

 Cells were stimulated for 4 h, mean percentage of positive cells ± SD, n = 6.  

 Cells were stimulated for 8 h, mean percentage of positive cells, n = 2.  

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