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Table 3

Cytokine Secretion by CD8⁺ T Cell Subsets

Cells^*	IL-2	IFN-γ	TNF-α	IL-4
	U/ml	pg/ml	pg/ml	pg/ml
CD45RA⁺CD27⁺	100	2,860	370	<1
CD45RA⁺CD27⁻	<10	9,310	2,220	<1
CD45RA⁻CD27⁺	100	11,940	1,660	10

Cells^*	IL-2	IFN-γ	TNF-α	IL-4
	U/ml	pg/ml	pg/ml	pg/ml
CD45RA⁺CD27⁺	100	2,860	370	<1
CD45RA⁺CD27⁻	<10	9,310	2,220	<1
CD45RA⁻CD27⁺	100	11,940	1,660	10

Sorted cells (2.5 × 10⁴) were stimulated with PMA (100 ng/ml) and ionomycin (1 μM) for 4 d. Cytokine content of the supernatants was tested with a bioassay for IL-2 and ELISAs for IFN-γ, TNF-α, and IL-4.

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