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Table 3

Cytokine Secretion by CD8+ T Cell Subsets

Cells*IL-2IFN-γTNF-αIL-4
  U/ml  pg/ml  pg/ml  pg/ml 
CD45RA+CD27+  100   2,860     370  <1 
CD45RA+CD27  <10   9,310  2,220  <1 
CD45RACD27+  100  11,940  1,660   10 
Cells*IL-2IFN-γTNF-αIL-4
  U/ml  pg/ml  pg/ml  pg/ml 
CD45RA+CD27+  100   2,860     370  <1 
CD45RA+CD27  <10   9,310  2,220  <1 
CD45RACD27+  100  11,940  1,660   10 
*

 Sorted cells (2.5 × 104) were stimulated with PMA (100 ng/ml) and ionomycin (1 μM) for 4 d. Cytokine content of the supernatants was tested with a bioassay for IL-2 and ELISAs for IFN-γ, TNF-α, and IL-4.  

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