Skip to Main Content
Table 1

Phagocytic Activity of the Three FcγR Classes Expressed in COS Cells

COS transfectantPhase and fluorescenceConfocal
RA*PIPI
Cytochalasin DCytochalasin D
++
Mock      
FcγRIA  20 ± 3  16 ± 1   61 ± 39  1 ± 1  28 ± 6 
FcγRIA tailless  18 ± 3  19 ± 2   40 ± 25  3 ± 1  ND 
FcγRIA + γ chain  10 ± 2  11 ± 4  270 ± 35  2 ± 1  135 ± 56 
FcγRIA tailless + γ chain  16 ± 4  15 ± 4  400 ± 133  4 ± 1  ND 
FcγRIIA  16 ± 2  16 ± 3  277 ± 152  3 ± 2  244 ± 81 
FcγRIIIA + γ chain  11 ± 2   9 ± 1  235 ± 68   178 ± 95 
COS transfectantPhase and fluorescenceConfocal
RA*PIPI
Cytochalasin DCytochalasin D
++
Mock      
FcγRIA  20 ± 3  16 ± 1   61 ± 39  1 ± 1  28 ± 6 
FcγRIA tailless  18 ± 3  19 ± 2   40 ± 25  3 ± 1  ND 
FcγRIA + γ chain  10 ± 2  11 ± 4  270 ± 35  2 ± 1  135 ± 56 
FcγRIA tailless + γ chain  16 ± 4  15 ± 4  400 ± 133  4 ± 1  ND 
FcγRIIA  16 ± 2  16 ± 3  277 ± 152  3 ± 2  244 ± 81 
FcγRIIIA + γ chain  11 ± 2   9 ± 1  235 ± 68   178 ± 95 

COS cells were incubated in the presence or absence of cytochalasin D with FITC-labeled RBCs opsonized with IgG for 1 h and were subjected to a brief hypotonic lysis to remove external RBCs, and were then fixed. 300 cells were examined per condition by phase and fluorescence microscopy and were scored for the number of phagocytosed RBCs. Data represent the mean and SD of three experiments.  

*

 RA (rosetting activity) was determined from cells not subjected to hypotonic lysis and is defined as the percentage of cells that bound three or more RBCs.  

 PI is defined as the number of RBCs ingested by 100 FcγR-expressing COS cells.  

§

 Confocal PI is the PI derived from three experiments analyzed by confocal microscopy.  

Close Modal

or Create an Account

Close Modal
Close Modal