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Table 3

Cupping Is Not Inhibited by Cytochalasin D in COS Cells

COS transfectantCytochalasin D +/−Distribution of beads by location: % of totalTotal No. of beads associated with cells
SurfaceCuppedSealed
FcγRIA  −  36  63   1.5   80 
FcγRIA   46  54        0   95 
FcγRIA + γ chain  −  11  59   30  148 
FcγRIA + γ chain   35  62    3  107 
FcγRIIA  −  76   4   20   25 
FcγRIIA   83  17    0   53 
COS transfectantCytochalasin D +/−Distribution of beads by location: % of totalTotal No. of beads associated with cells
SurfaceCuppedSealed
FcγRIA  −  36  63   1.5   80 
FcγRIA   46  54        0   95 
FcγRIA + γ chain  −  11  59   30  148 
FcγRIA + γ chain   35  62    3  107 
FcγRIIA  −  76   4   20   25 
FcγRIIA   83  17    0   53 

COS transfectants were incubated with IgG-coated latex beads with or without 1 μg/ml Cytochalasin D at 37°C for 1 h. Cells were processed as described in Materials and Methods, and were viewed by electron microscopy. The data sort into three categories, described in the legend to Table 2, that define the position of the bead relative to the COS cell membrane.  

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