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Table 4

Analysis of F-actin Polymerization following IgG-coated Bead Binding

COS transfectantBeads triggering F-actinTotal No. of bound beadsFold increase in F-actin staining intensity*
No. %
FcγRIA    6   1  432  3 ± 1 
FcγRIIA  104  45  230  9 ± 4 
COS transfectantBeads triggering F-actinTotal No. of bound beadsFold increase in F-actin staining intensity*
No. %
FcγRIA    6   1  432  3 ± 1 
FcγRIIA  104  45  230  9 ± 4 

COS cells were incubated with IgG-coated beads for 15 min at 37°C and were fixed and then stained with FITC-phalloidin to detect actin filaments. The cells were analyzed by confocal microscopy and F-actin staining subjacent to bound beads was analyzed for the presence of F-actin extensions around target beads. A 4-μm area beneath bound beads was also measured for F-actin staining intensity and was compared against background cortical F-actin of the same cell to determine a fold increase value. The data are derived from 35 cells for each condition from two experiments. The increase in F-actin staining intensity observed for FcγRIIA compared to FcγRIA is significant (P = 0.02).  

*

 Fold increase value derived only from beads which triggered F-actin accumulation.  

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