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Table 1

Effects of TGF-β1 on CD1a, E-cadherin, CLA, and Lag Expression

day 6
  day 0  GM-CSF + IL-4  GM-CSF + IL-4 + anti–TGF-β  GM-CSF + IL-4 + TGF-β1 
Cell number (× 105/ml  10  7.225 ± 0.6  6.35 ± 0.8  7.25 ± 0.5 
% CD1a-positive cells ± SD  <1  89.25 ± 3      91 ± 3      93 ± 4 
% E-cad-positive cells ± SD  <1   11.1 ± 6   6.2 ± 5      82 ± 8 
% CLA-positive cells  ND  <10  <10  80–90 
% Lag-positive cells   0   ND  20–40 
day 6
  day 0  GM-CSF + IL-4  GM-CSF + IL-4 + anti–TGF-β  GM-CSF + IL-4 + TGF-β1 
Cell number (× 105/ml  10  7.225 ± 0.6  6.35 ± 0.8  7.25 ± 0.5 
% CD1a-positive cells ± SD  <1  89.25 ± 3      91 ± 3      93 ± 4 
% E-cad-positive cells ± SD  <1   11.1 ± 6   6.2 ± 5      82 ± 8 
% CLA-positive cells  ND  <10  <10  80–90 
% Lag-positive cells   0   ND  20–40 

Anti–TGF-β1 blocking antibody was used at a concentration (20 μg/ml) that inhibits as high as 10 ng of recombinant TGF-β1. CD1a and E-cadherin expressions were determined by flow cytometry analysis; CLA and Lag expressions were determined by immunocytochemistry. Results are the mean (±SD) of four experiments.  

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