Rockefeller University Press

Table 1

Tetrameric Mamu-A^*01/p11C, C–M Complex Binding to T Cells Correlates with p11C-specific CTL Activity^*

Monkey	Percent CD8α/β⁺ T cells stained with Mamu-A^*01/p11C, C–M^‡			p11C-specific lysis^§ (Percentage)		pCTL^¶ (per 10⁶ cells)
Monkey	Whole blood	Con A/IL-2 expanded	p11C-pulsed APC expanded	Con A/IL-2 expanded (80.1)^‖	p11C-pulsed APC expanded (10.1)^‖	pCTL^¶ (per 10⁶ cells)
403	10.3	22.4	86.3	73	74	279
138	2.1	2.1	25.8	31	69	46
297	0.9	1.5	37.2	28	61	<3
348	0.7	0.5	5.1	22	15	<3

Monkey	Percent CD8α/β⁺ T cells stained with Mamu-A^*01/p11C, C–M^‡			p11C-specific lysis^§ (Percentage)		pCTL^¶ (per 10⁶ cells)
Monkey	Whole blood	Con A/IL-2 expanded	p11C-pulsed APC expanded	Con A/IL-2 expanded (80.1)^‖	p11C-pulsed APC expanded (10.1)^‖	pCTL^¶ (per 10⁶ cells)
403	10.3	22.4	86.3	73	74	279
138	2.1	2.1	25.8	31	69	46
297	0.9	1.5	37.2	28	61	<3
348	0.7	0.5	5.1	22	15	<3

p11C, C–M represents the optimal 9–amino acid fragment of the SIV Gag 12–amino acid peptide p11C (12).

‡

Staining with FITC-coupled tetrameric Mamu-A*01/p11C, C–M complex on gated CD8α/β T cells.

Percent p11C-specific lysis was calculated as percent specific release by p11C-pulsed MHC class I–matched target cells minus the average of the percent specific release by control peptide p11B-pulsed target cells.

‖

E/T ratio.

CTL precursor frequency for p11C per 10⁶ PBMCs assessed by LDA.

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