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Table 1

Effect of DNA Vaccination on Clinical and Histopathological Signs of EAE

ExperimentTreatmentn
Clinical dataHistopathology
Mean accumulated EAE scorePMean maximum EAE scorePMean inflammatory indexP
 pZZ/MBP68–85    3.4  0.0001  1.1  0.02     
  pZZ   15.2    2.6       
 pZZ/MBP68–85    4.5  0.001  0.8  0.028     
  pZZ   14.6    2.6       
  pZZ/MBP68–85   ND    ND    1.1  0.04 
  pZZ   ND    ND    4.0   
  pMBP68–85    9.1  NS  1.4  NS     
  pCIss   12.0    2.4       
  pZZ/MBP68–85*    5.4  0.006  0.6  0.028     
  pZZ*   13.6    2.1       
ExperimentTreatmentn
Clinical dataHistopathology
Mean accumulated EAE scorePMean maximum EAE scorePMean inflammatory indexP
 pZZ/MBP68–85    3.4  0.0001  1.1  0.02     
  pZZ   15.2    2.6       
 pZZ/MBP68–85    4.5  0.001  0.8  0.028     
  pZZ   14.6    2.6       
  pZZ/MBP68–85   ND    ND    1.1  0.04 
  pZZ   ND    ND    4.0   
  pMBP68–85    9.1  NS  1.4  NS     
  pCIss   12.0    2.4       
  pZZ/MBP68–85*    5.4  0.006  0.6  0.028     
  pZZ*   13.6    2.1       

The experiments were done on two occasions. Rats received the DNA vaccines 5 wk before induction of EAE with MBP68–85 in CFA. In experiment 2, 16 rats were killed on day 12 after immunization for evaluation of histopathology and immune responses. Mean accumulated EAE score is the cumulative disease score on day 21 after immunization (score obtained daily [0–4]). Mean maximum EAE score designates the average peak score of clinical disease at any time during the disease course, here on days 12–14 after immunization. Mean inflammatory index measures the degree of inflammation in spinal cord of rats killed on day 12 after immunization. P values were calculated with Mann-Whitney's test. NS, not significant at 0.05 level.  

*

 Rats were injected with mAb OX-8 on days 0, 7, and 13 after immunization to deplete CD8+ cells in vivo.  

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