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Table 1

Summary of In Vitro Differentiation of Th Cells

IL-4IL-15IL-10IFN-γ
Exp 1         
WT Th1 (%)    5    1    8  100 
WT Th2 (%)  100  100  100   10 
WT (%)    5   11   12   86 
6272 (%)    8   19   86   51 
6666 (%)  113   68  144   38 
Exp 2         
WT Th1 (%)    0    0    0  100 
WT Th2 (%)  100  100  100   80 
WT (%)    0    0    0  103 
6272 (%)    0    0   12  121 
6666 (%)   14   88   99   82 
Exp 3         
WT Th1 (%)   23   36   11  100 
WT Th2 (%)  100  100  100   28 
WT (%)   15   39    9  119 
6272 (%)   28   40   33   63 
6666 (%)  214  114   93   51 
Three independent experiments using in vitro differentiation of Th cells were performed as described in Fig. 3. The levels of IFN-γ obtained from wild-type Th1 (WT Th1) cells and the levels of Th2 cytokines (IL-4, IL-5, and IL-10) obtained from wild-type Th2 (WT Th2) cells were arbitrarily normalized to 100%. The actual concentration of cytokines from experiment 1 is shown in Fig. 3. 
IL-4IL-15IL-10IFN-γ
Exp 1         
WT Th1 (%)    5    1    8  100 
WT Th2 (%)  100  100  100   10 
WT (%)    5   11   12   86 
6272 (%)    8   19   86   51 
6666 (%)  113   68  144   38 
Exp 2         
WT Th1 (%)    0    0    0  100 
WT Th2 (%)  100  100  100   80 
WT (%)    0    0    0  103 
6272 (%)    0    0   12  121 
6666 (%)   14   88   99   82 
Exp 3         
WT Th1 (%)   23   36   11  100 
WT Th2 (%)  100  100  100   28 
WT (%)   15   39    9  119 
6272 (%)   28   40   33   63 
6666 (%)  214  114   93   51 
Three independent experiments using in vitro differentiation of Th cells were performed as described in Fig. 3. The levels of IFN-γ obtained from wild-type Th1 (WT Th1) cells and the levels of Th2 cytokines (IL-4, IL-5, and IL-10) obtained from wild-type Th2 (WT Th2) cells were arbitrarily normalized to 100%. The actual concentration of cytokines from experiment 1 is shown in Fig. 3. 
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