Activation of MAPK Isoforms in Wild-type and lyn−/− B Cells
| Genotype . | . | Stimulus . | . | n-Fold activation compared to unstimulated cells . | ||||
|---|---|---|---|---|---|---|---|---|
| MAPK isoform . | ||||||||
| Erk2 . | . | JNK1/2 . | . | p38 . | ||||
| C57BL/6 | anti-RP-105 | 21 ± 6 | 10 ± 2 | 6 ± 1 | ||||
| anti-IgM | 6 ± 2 | 3 ± 0.8 | 3 ± 1 | |||||
| Lyn−/− | anti-RP-105 | 6 ± 1 | 3 ± 0.8 | 2 ± 0.2 | ||||
| anti-IgM | 23 ± 9 | 14 ± 3 | 4 ± 1 | |||||
| Genotype . | . | Stimulus . | . | n-Fold activation compared to unstimulated cells . | ||||
|---|---|---|---|---|---|---|---|---|
| MAPK isoform . | ||||||||
| Erk2 . | . | JNK1/2 . | . | p38 . | ||||
| C57BL/6 | anti-RP-105 | 21 ± 6 | 10 ± 2 | 6 ± 1 | ||||
| anti-IgM | 6 ± 2 | 3 ± 0.8 | 3 ± 1 | |||||
| Lyn−/− | anti-RP-105 | 6 ± 1 | 3 ± 0.8 | 2 ± 0.2 | ||||
| anti-IgM | 23 ± 9 | 14 ± 3 | 4 ± 1 | |||||
Enzymatic activation of MAPK isoforms was determined after 15 min of B cell stimulation as described in the legend to Fig. 5. Enzymatic activity in resting wild-type cells was given an arbitrary value of 1. In each experiment splenic B cells isolated from at least four mice were used. The n-fold induction of kinase activity (mean ± standard error) from three to five separate experiments are presented.