TCR Vα Usage in Mutant TCR-β Transgenic Mice
. | . | Percent of CD4+ . | . | Percent of CD8+ . | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Vα2+ . | . | Vα3.2+ . | . | Vα8+ . | . | Vα11+ . | Vα2+ . | . | Vα3.2+ . | . | Vα8+ . | . | Vα11+ . | |||
β | 22.7 ± 1.0 | 0.7 ± 0.2 | 3.0 ± 0.1 | 1.5 ± 0.4 | 7.7 ± 1.8 | 1.0 ± 0.1 | 4.5 ± 0.1 | 1.2 ± 0.2 | ||||||||
β-loop− | 22.7 ± 0.9 | 0.5 ± 0.1 | 2.6 ± 0.3 | 2.3 ± 0.3 | 7.6 ± 1.1 | 1.4 ± 0.6 | 3.6 ± 0.7 | 2.0 ± 0.3 |
. | . | Percent of CD4+ . | . | Percent of CD8+ . | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Vα2+ . | . | Vα3.2+ . | . | Vα8+ . | . | Vα11+ . | Vα2+ . | . | Vα3.2+ . | . | Vα8+ . | . | Vα11+ . | |||
β | 22.7 ± 1.0 | 0.7 ± 0.2 | 3.0 ± 0.1 | 1.5 ± 0.4 | 7.7 ± 1.8 | 1.0 ± 0.1 | 4.5 ± 0.1 | 1.2 ± 0.2 | ||||||||
β-loop− | 22.7 ± 0.9 | 0.5 ± 0.1 | 2.6 ± 0.3 | 2.3 ± 0.3 | 7.6 ± 1.1 | 1.4 ± 0.6 | 3.6 ± 0.7 | 2.0 ± 0.3 |
Lymph node cells from TCR-β (β) or mutant TCR-β (β-loop−) transgenic mice were triple stained with the FITC-labeled anti-Vα Abs, anti-CD4– PE, and anti-CD8–Cy5. Results (three mice per group) are expressed as the percentage of stained cells ± SD.