Mean Fold Induction of the IL-1Rα and IL-1β Genes in Response to M. tuberculosis
| . | . | Haplotype . | . | Number . | . | Fold induction* . | . | Range . |
|---|---|---|---|---|---|---|---|---|
| IL-1Ra | ||||||||
| A2− | 5 | 5.7 | 1.7–8.6 | |||||
| A2+ | 8 | 10.0 | 2.6–29.5 | |||||
| IL-1β (−511) | ||||||||
| A2− | 3 | 25.9 | 11.3–51.0 | |||||
| A2+ | 10 | 46.5 | 23.7–84.7 | |||||
| IL-1β (+3953) | ||||||||
| A2− | 7 | 52.3 | 15.5–84.7 | |||||
| A2+ | 6 | 29.3 | 11.3–47.5 |
| . | . | Haplotype . | . | Number . | . | Fold induction* . | . | Range . |
|---|---|---|---|---|---|---|---|---|
| IL-1Ra | ||||||||
| A2− | 5 | 5.7 | 1.7–8.6 | |||||
| A2+ | 8 | 10.0 | 2.6–29.5 | |||||
| IL-1β (−511) | ||||||||
| A2− | 3 | 25.9 | 11.3–51.0 | |||||
| A2+ | 10 | 46.5 | 23.7–84.7 | |||||
| IL-1β (+3953) | ||||||||
| A2− | 7 | 52.3 | 15.5–84.7 | |||||
| A2+ | 6 | 29.3 | 11.3–47.5 |
Freshly isolated monocytes from 13 donors of differing genotypes were rested overnight and then stimulated for 4 h with M. tuberculosis at 1:1. Cytokine gene expression was quantitated by hybridization of 2 μg of the resultant RNA to [32P]UTP-labeled complimentary RNA probes, including L32 and GAPDH as constitutively expressed “housekeeping” genes. The M. tuberculosis–induced fold increase in IL-1Ra or IL-1β gene expression was calculated by dividing the band density in the presence of M. tuberculosis by the density in its absence.
P values were 0.30 for IL-1Ra, 0.14 for IL-1β (−511), and 0.04 for IL-1β (+3953).