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Table III

IFN-α/β Do Not Raise Bcl-2 Levels in Activated T Cells

Culture conditionsPercentage of Vβ8+ cells aliveBcl-2 levels in
Vβ8+ cells withVβ6+ cells with
CD4CD8CD4CD8CD4CD8
No Addition  33.5 ± 0.4  11.9 ± 0.3   9.1 ± 2.1   8.2 ± 1.2  13.0 ± 1.6  20.6 ± 2.5 
IFN-α/β  48.0 ± 2.3  48.9 ± 1.0   7.1 ± 1.3   7.9 ± 1.4  13.3 ± 1.6  24.7 ± 1.3 
IL-2  73.3 ± 4.3  73.3 ± 4.2  18.1 ± 2.1  36.5 ± 1.5  15.0 ± 1.5  39.0 ± 2.7 
IL-15  80.7 ± 2.2  63.9 ± 2.7  23.8 ± 3.7  37.8 ± 3.2  18.9 ± 2.8  49.9 ± 8.5 
Culture conditionsPercentage of Vβ8+ cells aliveBcl-2 levels in
Vβ8+ cells withVβ6+ cells with
CD4CD8CD4CD8CD4CD8
No Addition  33.5 ± 0.4  11.9 ± 0.3   9.1 ± 2.1   8.2 ± 1.2  13.0 ± 1.6  20.6 ± 2.5 
IFN-α/β  48.0 ± 2.3  48.9 ± 1.0   7.1 ± 1.3   7.9 ± 1.4  13.3 ± 1.6  24.7 ± 1.3 
IL-2  73.3 ± 4.3  73.3 ± 4.2  18.1 ± 2.1  36.5 ± 1.5  15.0 ± 1.5  39.0 ± 2.7 
IL-15  80.7 ± 2.2  63.9 ± 2.7  23.8 ± 3.7  37.8 ± 3.2  18.9 ± 2.8  49.9 ± 8.5 

Lymph node T cells were purified from C57BL/10 mice given SEB 2 d previously. At that time, levels of Bcl-2 were 17.8 ± 0.6 and 17.6 ± 0.9 in Vβ8 cells bearing CD4 and CD8, respectively, and 28.2 ± 0.9 and 38.7 ± 0.9 in Vβ6 cells bearing CD4 and CD8, respectively. Cells were cultured for 24 h alone or with 3,333 U/ml IFN-α/β or 100 ng/ml IL-2 or 100 ng/ml IL-15 and then assayed for intracellular amounts of Bcl-2 as described in Materials and Methods. Results shown are the mean ± SE of staining with anti–Bcl-2 antibody in arbitrary units based on staining intensities of three identical cultures minus the background without primary antibody.  

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