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Table IV

IFN-α/β Do Not Raise Bcl-X Levels in Activated T Cells

Culture conditionsBcl-X levels in
Vβ8+ cells bearingVβ6+ cells bearing
CD4CD8CD4CD8
No addition  23.0 ± 2.2  29.0 ± 1.7  21.6 ± 2.5  32.0 ± 5.5 
IFN-α/β  25.2 ± 2.9  27.7 ± 4.4  22.1  24.1 ± 3.7 
IL-2  25.7 ± 2.8  28.3 ± 0.5  20.3 ± 2.8  25.7 ± 2.3 
IL-15  30.3 ± 1.0  46.2 ± 6.1  23.2 ± 4.4  46.5 ± 4.0 
Culture conditionsBcl-X levels in
Vβ8+ cells bearingVβ6+ cells bearing
CD4CD8CD4CD8
No addition  23.0 ± 2.2  29.0 ± 1.7  21.6 ± 2.5  32.0 ± 5.5 
IFN-α/β  25.2 ± 2.9  27.7 ± 4.4  22.1  24.1 ± 3.7 
IL-2  25.7 ± 2.8  28.3 ± 0.5  20.3 ± 2.8  25.7 ± 2.3 
IL-15  30.3 ± 1.0  46.2 ± 6.1  23.2 ± 4.4  46.5 ± 4.0 

Lymph node T cells were purified from C57BL/10 mice given SEB 2 d previously. At that time Bcl-X levels were 51.3 ± 1.9 and 49.9 ± 1.9 in Vβ8+ cells bearing CD4 and CD8, respectively, and 35.5 ± 0.9 and 37.9 ± 1.3 in Vβ6+ cells bearing CD4 and CD8, respectively. Cells were cultured for 24 h alone or with 3,333 U/ml IFN-α/β or 100 ng/ml IL-2 or IL-15 and then assayed for intracellular amounts of Bcl-X as described in Materials and Methods. Results shown are the mean ± SE of three identical cultures in arbitrary units of Bcl-X based on staining intensities minus the background without primary antibody. Cell survival rates were as listed in Table III.  

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