All infected foci in 50 consecutive ×63 microscopic fields were scored as follows for granuloma formation: none, single or fused parasitized Kupffer cells with no mononuclear cell infiltrate; developing, core of fused infected Kupffer cells with some cell infiltrate; mature, fused Kupffer cell core surrounded by a well-developed mononuclear cell mantle; and empty, granuloma devoid of amastigotes (1–4). See Figs. 2 and 3. Results are from two experiments, and indicate mean values for four to five mice at each time point. SEM, omitted for clarity, were all <12%.