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Table I

Recognition of Mutated TPI by CD4+ TIL 1558

Stimulatorcell lysate
Transfected gene
cDNA sequence,nucleotide 450
GM-CSF
      pg/ml 
1558-mel  None  T, C  6,315 
1558-EBV  None   <8 
COS-7  TPI-1B   3,485 
COS-7  TPI-5B   8,310 
COS-7  TPI-11G   6,340 
COS-7  TPI-4B   
COS-7  TPI-12A   11 
COS-7  TPI-12F   11 
COS-7  β-actin  n.a.  <8 
Stimulatorcell lysate
Transfected gene
cDNA sequence,nucleotide 450
GM-CSF
      pg/ml 
1558-mel  None  T, C  6,315 
1558-EBV  None   <8 
COS-7  TPI-1B   3,485 
COS-7  TPI-5B   8,310 
COS-7  TPI-11G   6,340 
COS-7  TPI-4B   
COS-7  TPI-12A   11 
COS-7  TPI-12F   11 
COS-7  β-actin  n.a.  <8 

COS-7 cells were transfected for 4 d with the indicated genes. Cell lysates were then incubated with THP-1 monocytic leukemia cells for 20 h, after which TIL were added for 20 h. GM-CSF secretion by TIL was measured by ELISA. n.a., not applicable. 

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