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Table II

Determining the PI Anchor Residue for TPImut 23–37

TPImut residuesSequenceGM-CSF
    pg/ml 
23–37                  G E L I G I L N A A K V P A D  25,390 
24–37            E L I G I L N A A K V P A D  28,460 
25–37              L I G I L N A A K V P A D  11,750 
26–38                I G I L N A A K V P A D T   7,210 
27–39                 G I L N A A K V P A D T E  38 
28–40                   I L N A A K V P A D T E V   22 
29–41                     L N A A K V P A D T E V V  34 
20–34  Q S L G E L I G I L N A A K V  5,960 
20–33  Q S L G E L I G I L N A A K  35 
20–32  Q S L G E L I G I L N A A  25 
20–31  Q S L G E L I G I L N A  24 
TPImut residuesSequenceGM-CSF
    pg/ml 
23–37                  G E L I G I L N A A K V P A D  25,390 
24–37            E L I G I L N A A K V P A D  28,460 
25–37              L I G I L N A A K V P A D  11,750 
26–38                I G I L N A A K V P A D T   7,210 
27–39                 G I L N A A K V P A D T E  38 
28–40                   I L N A A K V P A D T E V   22 
29–41                     L N A A K V P A D T E V V  34 
20–34  Q S L G E L I G I L N A A K V  5,960 
20–33  Q S L G E L I G I L N A A K  35 
20–32  Q S L G E L I G I L N A A  25 
20–31  Q S L G E L I G I L N A  24 

THP-1 cells pretreated with IFN-γ were pulsed overnight with the indicated peptides at 100 nM, then T cells were added for 20 h. GM-CSF secretion by T cells was measured by ELISA. Background secretion of T cells plus THP-1 without peptide = 28 pg/ml. 

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