Increased Cytokine Production by Txk Transgenic CD4+ Lymphocytes
| Cell type . | Treatment . | IL-2 . | IL-4 . | IFN-γ . |
|---|---|---|---|---|
| pg/ml | pg/ml | pg/ml | ||
| wt | Control | ND | ND | ND |
| txk tg | Control | ND | ND | ND |
| txk tg/tg | Control | ND | ND | ND |
| wt | Anti-CD3 | ND | 8 | 1,134 |
| txk tg | Anti-CD3 | 12 | 52 | 7,193 |
| txk tg/tg | Anti-CD3 | 56 | 65 | 8,245 |
| wt | Anti-CD3 plus anti-CD28 | 24 | 34 | 19,258 |
| txk tg | Anti-CD3 plus anti-CD28 | 2,994 | 120 | 43,780 |
| txk tg/tg | Anti-CD3 plus anti-CD28 | 6,064 | 146 | 26,435 |
| Cell type . | Treatment . | IL-2 . | IL-4 . | IFN-γ . |
|---|---|---|---|---|
| pg/ml | pg/ml | pg/ml | ||
| wt | Control | ND | ND | ND |
| txk tg | Control | ND | ND | ND |
| txk tg/tg | Control | ND | ND | ND |
| wt | Anti-CD3 | ND | 8 | 1,134 |
| txk tg | Anti-CD3 | 12 | 52 | 7,193 |
| txk tg/tg | Anti-CD3 | 56 | 65 | 8,245 |
| wt | Anti-CD3 plus anti-CD28 | 24 | 34 | 19,258 |
| txk tg | Anti-CD3 plus anti-CD28 | 2,994 | 120 | 43,780 |
| txk tg/tg | Anti-CD3 plus anti-CD28 | 6,064 | 146 | 26,435 |
IL-2, IL-4, and IFN-γ production were determined by ELISA from CD4+ lymphocytes from wild-type (wt), txk homozygous transgenic (tg/tg), and txk hemizygous transgenic (tg) mice. Lymphocytes were plated under control conditions or with plate-bound anti-CD3 or plate-bound anti-CD3 and anti-CD28. Supernatants were analyzed after 24 (IL-4) or 48 (IL-2, IFN-γ) h. The results are representative of three experiments. ND, none detected.