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Table I.

Increased Cytokine Production by Txk Transgenic CD4+ Lymphocytes

Cell typeTreatmentIL-2IL-4IFN-γ
  pg/ml pg/ml pg/ml 
wt Control ND ND ND 
txk tg Control ND ND ND 
txk tg/tg Control ND ND ND 
wt Anti-CD3 ND 1,134 
txk tg Anti-CD3 12 52 7,193 
txk tg/tg Anti-CD3 56 65 8,245 
wt Anti-CD3 plus anti-CD28 24 34 19,258 
txk tg Anti-CD3 plus anti-CD28 2,994 120 43,780 
txk tg/tg Anti-CD3 plus anti-CD28 6,064 146 26,435 
Cell typeTreatmentIL-2IL-4IFN-γ
  pg/ml pg/ml pg/ml 
wt Control ND ND ND 
txk tg Control ND ND ND 
txk tg/tg Control ND ND ND 
wt Anti-CD3 ND 1,134 
txk tg Anti-CD3 12 52 7,193 
txk tg/tg Anti-CD3 56 65 8,245 
wt Anti-CD3 plus anti-CD28 24 34 19,258 
txk tg Anti-CD3 plus anti-CD28 2,994 120 43,780 
txk tg/tg Anti-CD3 plus anti-CD28 6,064 146 26,435 

IL-2, IL-4, and IFN-γ production were determined by ELISA from CD4+ lymphocytes from wild-type (wt), txk homozygous transgenic (tg/tg), and txk hemizygous transgenic (tg) mice. Lymphocytes were plated under control conditions or with plate-bound anti-CD3 or plate-bound anti-CD3 and anti-CD28. Supernatants were analyzed after 24 (IL-4) or 48 (IL-2, IFN-γ) h. The results are representative of three experiments. ND, none detected.

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